朱砂叶螨为害前后抗、感木薯品种叶组织营养物质含量差异分析

Analysis on Difference of Nutrient Content in Leaf Tissue of Resistant and Sensitive Cassava Varieties Before and After Damage to Tetranychus cinnabarinus

为了明确营养物质在木薯抗螨性中的重要作用,在获得遗传稳定的抗、感螨参照标准木薯品种的基础上,采用分光光度法开展了朱砂叶螨为害前后抗、感螨参照标准木薯品种叶组织中营养物质含量的差异分析。结果表明:朱砂叶螨为害1、4、8 d的抗螨参照标准木薯品种C1115叶组织中的游离氨基酸(FAA)、可溶性糖(WSS)、可溶性氮(SN)含量均显著降低,分别是为害前的69.69%、69.29%和63.42%,73.16%、73.71%和72.75%,45.97%、46.21%和46.13%(P<0.05);但糖氮比(S/N)显著升高,是为害前的159.15%、159.46%和157.83%(P<0.05),游离脯氨酸(Pro)含量无显著变化。螨害1、4、8 d的感螨参照标准木薯品种BRA900叶组织中的FAA含量和WSS含量与为害前无显著差异,SN含量显著升高,是为害前的148.50%、148.73%和148.77%;S/N显著降低,是为害前的62.61%、61.48%和61.92%;Pro含量显著升高,是为害前的373.82%、367.33%和362.61%,但为害前后无显著性差异(P<0.05)。

The difference of nutrient contents in leaf tissues of resistant and susceptible standard cassava varieties before and after being damaged by Tetranychus cinnabarinus was analyzed by spectrophotometry.The content of free amino acids(FAA),soluble sugars(WSS),soluble nitrogen(SN)in the leaves of C1115,a standard cassava cultivar for mite resistance,significantly decreased damaged by T.cinnabarinus.The content of FAA,WSS and SN 1,4 and 8 days after damaged by T.cinnabarinus was,69.69%,69.29%and 63.42%,73.16%,73.71%and 72.75%,45.97%,46.21%and 46.13%,respectively(P<0.05)of that before damaged.S/N increased significantly,which was 159.15%,159.46%and 157.83%1,4 and 8 days after damaged by T.cinnabarinus respectively(P<0.05)of that before damaged,while the content of free proline(Pro)did not change significantly.There was no significant difference in the content of FAA,WSS between the leaves of the mite-susceptible reference standard cassava variety BRA900 after damaged for 1,4 and 8 days.The content of SN increased significantly,which was 148.50%,148.73%and 148.77%1,4 and 8 days after damaged by Tetranychus cinnabarinus respectively of that before damaged.S/N decreased significantly,which was 62.61%,61.48%and 61.92%of that before damaged.The content of free proline(Pro)increased significantly,which was 373.82%,367.33%and 362.61%of that before damaged.