高核心岩藻糖基化母乳促进子代肠道双歧杆菌和乳杆菌的优势生长

High core fucosylated breast milk promotes the dominant growth of intestinal Bifidobacterium and Lactobacillus in neonates

目的探究母乳蛋白核心岩藻糖基化水平对新生儿肠道菌群结构的影响。方法采用凝集素(aspergillus oryzae lectin,AOL)检测不同母乳样本的蛋白核心岩藻糖基化水平,按照蛋白核心岩藻糖基化水平的高低将母乳样品分为高核心岩藻糖基化组和低核心岩藻糖基化组,采集两组母乳样品对应喂养的婴儿的粪便,利用PCR-变性梯度凝胶电泳(PCR-DGGE)的方法检测两组婴儿粪便中菌群的组成。利用Fut8^+/-和Fut8^+/+模型小鼠作为母代让其分别哺育野生型子代仔鼠,以进一步阐明核心岩藻糖基化对新生儿肠道菌群的影响。结果母亲/母鼠母乳蛋白岩藻糖基化水平高组与低组相比,婴儿肠道中双歧杆菌菌群丰富度增加,仔鼠肠道内乳杆菌定植增加。结论高核心岩藻糖基化的母乳可以促进子代肠道中双歧杆菌和乳杆菌等益生菌的生长。

Objective To explore the effect of core fucosylation level of breast milk protein on the structure of intestinal flora in newborns.Methods High-core fucosylated breast milk could promote the growth of probiotics such as Bifidobacterium and Lactobacillus in the intestinal tract of offspring.Lectin imprinting was used to detect the protein core fucosylation level of different breast milk samples.Breast milk samples were divided into two groups according to the level of protein core fucosylation.The fecal samples of neonates in the two groups were collected.PCR-denaturing gradient gel electrophoresis(PCR-DGGE)was used to detect the composition of the bacterial flora in the feces.Fut8^+/-and Fut8^+/+model mice were used as mother mice to feed wild-type offsprings,respectively,to further clarify the effect of core fucosylation on the intestinal flora of newborns.Results Compared with the low fucosylation level group,the abundance of Bifidobacterium flora in the intestinal tract of infants in the high fucosylation level group increased,so did the colonization of Lactobacillus in the intestine of offspring rats.Conclusion High core fucosylated breast milk can promote the growth of probiotics such as Bifidobacterium and Lactobacillus in the intestinal tract of offspring.