野西瓜乙醇提取物对大鼠细菌性阴道炎及阴道菌群的影响

Effect of ethanol extract of wild watermelon on bacterial vaginitis and vaginal flora in rats

目的观察野西瓜乙醇提取物对细菌性阴道炎及阴道菌群的影响。方法建立细菌性阴道炎模型并分为模型组、对照组、低剂量组、中剂量组和高剂量组,另取8只正常SD大鼠记为正常组。对照组予以氧氟沙星60 mg/kg灌胃,低剂量组、中剂量组和高剂量组分别予以50、100、200 mg/kg西瓜乙醇提取物灌胃,模型组与正常组均予以等量无菌生理盐水灌胃。对比疗效、干预前后阴道菌群变化。结果总有效率高剂量组最高,中剂量组和对照组次之,低剂量组较低,除中剂量组和对照组差异无统计学意义外(P>0.05),其余两两比较差异均有统计学意义(均P<0.05);干预前模型组、对照组和三个剂量组阴道菌群Chao1指数、Shannon指数、厚壁菌门、放线菌门、乳杆菌科、双歧杆菌科、真杆菌科、乳杆菌属、双歧杆菌属、优杆菌属水平相对丰度均低于正常组,干预后模型组均下降,对照组和三个剂量组均升高,且干预后模型组均低于正常组,对照组和三个剂量组均高于模型组,除中剂量组和对照组差异均无统计学意义外(P>0.05),其余两两比较差异均有统计学意义(均P<0.05);阴道变形菌门、子囊菌门和半知菌亚门相对丰度、葡萄球菌科、假单胞菌科、肠杆菌科、酵母科、丛梗孢科水平相对丰度、葡萄球菌属、假单胞菌属、大肠杆菌属、假丝酵母菌属、曲霉菌属相对丰度与上述变化正相反;野西瓜乙醇提取物的作用呈剂量依赖性。结论野西瓜乙醇提取物对大鼠细菌性阴道炎治疗效果好,呈剂量依赖性,并可调节阴道菌群。

Objective To observe the effect of ethanol extract of wild watermelon on bacterial vaginitis(BV)and vaginal flora in rats.Methods BV models were established and divided into the model group,control group,low dose group,medium dose group or high dose group,with another 8 normal SD rats as the normal group.The control group was given 60 mg/kg ofloxacin by gavage,the low dose group,medium dose group and high dose group were given 50 mg/kg,100 mg/kg and 200 mg/kg watermelon ethanol extract by gavage,while the model group and normal group were given the same amount of sterile saline by gavage.The efficacy and changes of vaginal flora before and after intervention were compared among groups.Results The total efficiency rate in high dose group was the highest,followed by the control group and medium dose group,and lowest in the low dose group;pairwise comparisons showed significant differences among groups(all P<0.05)but not between the medium dose group and control group(P>0.05).Before intervention,the Chao1 index,Shannon index and relative abundances of Phylum Firmicutes,Phylum Actinobacteria,Lactobacillaceae,Bifidobacteriaceae,Eubacteriaceae,Lactobacillus,Bifidobacterium and Eubacterium in model group,control group and the 3 dose groups were lower than those in normal group(all P<0.05)respectively,which after intervention decreased in model group while increased in control group and the 3 dose groups,with those in model group being lower than in normal group,and those in control group and the 3 dose groups being higher than in the model group;and pairwise comparisons showed no significant difference between the medium dose group and control group(P>0.05),but between the other paired groups(all P<0.05).The changes in the relative abundances of Proteobacteria,Ascomycota,Deuteromycotina,Staphylococcaceae,Pseudomonadaceae,Enterobacteriaceae,Saccharomycetaceae,Moniliaceae,Staphylococcus,Pseudomonas,Coliform,Candida and Aspcrgillus were contrary to the above changes.The effect of ethanol extract of wild watermelon was dose-dependent.Conclusion The ethanol extract of wild watermelon has a good effect on bacterial vaginitis in a dose-dependent manner,and can regulate vaginal flora.