商品化糖苷酶转化糖苷类天然产物研究进展

Research Progresses on Converting Glycoside Natural Products by Commercial Glycosidases

糖苷类化合物广泛存在于药用植物中,具有多种潜在的生物活性。众多研究表明糖苷类化合物中糖单元对于活性具有至关重要的作用。相比于传统的化学方法,使用酶解法进行糖基转化,具有反应温和环保,特异性好等特点,可以实现糖基部分的选择性水解。但是不同实验室来源的糖基酶也具有基原复杂、比化学试剂质量稳定性差等劣势。因此,使用来源可控、性能相对稳定的商品化糖苷酶是开展糖苷类天然产物糖基选择性修饰的可行途径。以使用商品化酶水解中药天然产物的研究为契入点,对它们的特点和应用展开综述。

Glycoside compounds are widely present in medicinal plants and have a variety of potential biological activities.Numerous studies have shown that sugar units in glycoside compounds play an essential role in their biological activity.Compared with traditional chemical methods,enzymatic hydrolysis by glycosylases has characterized by mild condition,environmental-friendship,and high specificity and could be used for selective hydrolysis of sugar unit.However,glycosylases from different labs suffer from various origins,uncontrollable quality and stability issues in comparison with chemical reagents.Therefore,the use of commercially available glycosylases that originate from a stable source with quality control is a feasible way to carry out selective modification on the glycoside compounds.Toward to this end,this review summarized recent applications on converting glycosides in TCM natural products by commercially available glycosidases.