胶体金法和被动颗粒凝集法在儿童肺炎支原体感染检测中的应用比较

Comparison of colloidal gold method and passive particle agglutination method in the detection of Mycoplasma pneumoniae infection in children

目的比较胶体金法和被动颗粒凝集法检测肺炎支原体(MP)感染的符合率,探讨儿童中应用被动颗粒凝集法辅助临床诊断MP近期或现症感染的总抗体滴度阈值。方法选取2019年1-7月于该院呼吸科住院治疗的临床诊断为MP呼吸道感染患儿265例。使用被动颗粒凝集法检测265例患儿血清MP总抗体滴度,采用胶体金法检测血清MP-IgM,并对检测结果进行统计学分析。结果以患儿血清MP总抗体滴度≥1∶160作为MP近期或现症感染的诊断标准,两种方法的总符合率为81.89%,差异无统计学意义(P=0.193);若以总抗体滴度≥1∶80作为MP近期或现症感染的诊断标准,两种方法的总符合率为85.66%,差异有统计学意义(P=0.034)。总抗体滴度1∶80和1∶160组中MP-IgM阳性率分别为70.83%和71.70%,差异无统计学意义(P=0.938)。结论在儿童感染MP后,随着血清中MP总抗体滴度增加,MP-IgM阳性率增高;血清MP总抗体滴度≥1∶160和胶体金法血清MP-IgM阳性作为MP近期或现症感染的诊断标准,均可为临床提供可靠且一致性好的诊断依据。

Objective To compare the coincidence rate between colloidal gold method and passive particle agglutination method in detecting Mycoplasma pneumoniae(MP)infection,and to explore the total antibody titer threshold of passive particle agglutination in assisting clinical diagnosis of MP infection in children.Methods From January 2019 to July 2019,265 children with MP respiratory tract infection in respiratory department of our hospital were selected.The total MP antibody titers of 265 children were detected by passive particle agglutination method,and the serum MP-IgM was detected by colloidal gold method,and the results were statistically analyzed.Results If the antibody titer≥1∶160 was used as the diagnostic standard of MP recent or present infection,the total coincidence rate of the two methods was 81.89%with no statistically significant difference(P=0.193).If the antibody titer≥1∶80 was used as the diagnosis standard of MP recent or present infection,the total coincidence rate of the two methods was 85.66%,with statistically significant difference(P=0.034).In addition,the MP-IgM positive rate in the antibody titer 1∶80 and 1∶160 groups was 70.83%and 71.70%respectively,with no statistically significant difference(P=0.938).Conclusion After MP infection in children,the positive rate of MP-IgM increased with the increase of the total MP antibody titer in serum.As the diagnostic standard for recent or current MP infections,both serum MP total antibody titer≥1∶160 and serum MP-IgM positive by colloidal gold method could provide reliable and consistent diagnostic basis for clinical practice.