MnSOD、SIRT3在肺腺癌组织及癌旁组织中的表达及其与临床病理、预后的关系分析

Expression of MnSOD and SIRT3 in lung adenocarcinoma and adjacent tissues and its relationship with clinical pathology and prognosis

目的分析锰超氧化物歧化酶(MnSOD)、去乙酰化酶3(SIRT3)在肺腺癌组织及癌旁组织中的表达及其与临床病理、预后的关系。方法选取该院心胸血管外科2016年1月至2018年12月收治的60例肺腺癌患者,均留存有肺腺癌及癌旁组织标本。采用免疫组织化学(SP法)检测肺腺癌组织及癌旁组织MnSOD、SIRT3表达,比较其在不同病理特征患者肺腺癌组织MnSOD、SIRT3表达差异,对所有患者予以持续随访,采用Cox回归模型分析影响患者预后的独立危险因素,并绘制Kaplan-Meier生存曲线,分析MnSOD、SIRT3对肺腺癌患者生存时间的影响。结果肺腺癌组织中MnSOD阳性表达率、SIRT3阳性表达率明显低于癌旁组织,差异有统计学意义(P<0.05);不同性别、年龄、肺腺癌亚型及有无吸烟史的患者组织MnSOD、SIRT3表达差异无统计学意义(P>0.05),但随着组织学分级增加,肺腺癌组织MnSOD、SIRT3阳性表达率明显下降,TNM分期为Ⅲ期患者肺腺癌组织MnSOD、SIRT3阳性表达率明显低于Ⅰ+Ⅱ期患者,差异有统计学意义(P<0.05);Cox回归分析显示,MnSOD阴性、SIRT3阴性、组织学分级Ⅲ级、淋巴结转移、TNM分期Ⅲ期均是影响肺腺癌患者生存的独立危险因素;MnSOD、SIRT3阴性患者累积生存率明显低于MnSOD、SIRT3阳性患者,差异有统计学意义(P<0.05)。结论肺腺癌患者肺腺癌组织存在MnSOD、SIRT3低表达现象,并与组织学分级、淋巴结转移、TNM分期及生存密切相关。

Objective To analyze the expression of manganese-containing superoxide dismutase(MnSOD)and sirtuin 3(SIRT3)in lung adenocarcinoma and adjacent tissues and its relationship with clinical pathology and prognosis.Methods Sixty patients with lung adenocarcinoma who were admitted and treated in the department of cardiothoracic and vascular surgery in our hospital from January 2016 and December 2018 were selected.Their lung adenocarcinoma and adjacent tissue specimens were collected to detect the expression of MnSOD and SIRT3 by Immunohistochemistry method(SP method).The expression of MnSOD and SIRT3 in lung adenocarcinoma tissues of patients with different pathological characteristics was compared.All patients were followed up,and Cox regression model was used to analyze independent risk factors affecting the prognosis of patients.Besides,Kaplan-Meier survival curves were used to analyze effects of MnSOD and SIRT3 on the survival time of patients with lung adenocarcinoma.Results The positive expression rates of MnSOD and SIRT3 in lung adenocarcinoma tissues were significantly lower than those in adjacent tissues(P<0.05).There were no statistically significant differences on the expression of MnSOD and SIRT3 in the tissues of patients with different genders,ages,lung adenocarcinoma subtypes and patients with or without smoking history(P>0.05).With the increase of histological stage,the positive expression rates of MnSOD and SIRT3 in lung adenocarcinoma tissues decreased significantly.The positive expression rates of MnSOD and SIRT3 in lung adenocarcinoma tissues at TNM stageⅢwere significantly lower than those at stageⅠandⅡ(P<0.05).Cox regression analysis showed that MnSOD negative,SIRT3 negative,histological gradeⅢ,lymph node metastasis and TNM stageⅢwere independent risk factors affecting the survival of patients with lung adenocarcinoma.The cumulative survival rates of MnSOD and SIRT3 negative patients were significantly lower than those of MnSOD and SIRT3 positive ones(P<0.05).Conclusion Low expression of MnSOD and SIRT3 in lung adenocarcinoma tissues of patients with lung adenocarcinoma is closely related to histological grade,lymph node metastasis,TNM staging and survival.