游离骨移植在小鼠颅骨缺损中的成活机制研究

Survival mechanism of free bone graft in calvarial defects using a murine model

目的探讨游离骨移植在颅骨缺损中的成活机制。方法通过将4周龄绿色荧光蛋白(GFP)小鼠头颅骨移植到4周龄裸鼠颅骨缺损来模拟重建骨移植,实验分观察组、阳性对照组,每组15只。4~24周后进行大体观察,骨组织面积测量(BTA%)和绿色荧光量(GFP%)的检测。结果大体观察显示,观察组小鼠颅骨左侧缺损术后4~24周逐渐出现成骨,与周围骨组织紧密连接且具有相同的厚度。单纯颅骨缺损区域没有新骨形成,仅被纤维组织覆盖,表明缺损未被修复。组织学提示,观察组术后4、8、12、18、24周颅骨骨移植部位骨组织面积百分比BTA(%)值与阳性对照组差异无统计学意义(P>0.05)。绿色荧光量(GFP%)的检测,观察组术后4、8、12、18、24周为(6.33±0.73)%、(3.40±0.68)%、(1.98±0.21)%、(0.81±0.08)%、(0.68±0.06)%,各观察时间点的数据与阳性对照组比较,差异有统计学意义(P<0.05)。结论游离骨移植在颅骨缺损的成活机制主要是骨的爬行替代和骨再生。

Objective To study the mechanism of free bone graft survival in calvarial defects.Methods We simulated the bone autograft reconstruction through transplanting green fluorescent protein(GFP)mice'cranium to the nude mice'calvarial bone defects.Then gross and histological evaluation were performed to calculate BTA(%)and GFP(%)in graft bone.Results The results showed that the green fluorescence was getting disappeared from 4 to 24 weeks'post-operation.However,a small amount of green fluorescence remained at 24 weeks'post-operation(6.69%of the positive control group).In addition,the gross and histological evaluation indicated that the grafted bone survived well at different time points.Conclusions The survival mechanism of free bone graft in cranium mainly relies on creeping substitution assisted with bony regeneration.