藤茶黄酮绿色制备纳米氧化锌及其抗氧化和抗菌性能

Green synthesis of ZnO nanoparticles using flavone from Ampelopsis grossedentata and its antioxidant and antibacterial properties

以藤茶黄酮为原料,采用溶液燃烧法绿色制备纳米氧化锌(Nano ZnO)粉末,并利用FTIR、XRD、SEM和EDS对所得样品进行结构分析和形貌观察;使用布兰德-威廉姆斯法、透明抑菌圈法和最小抑菌浓度法考察了绿色合成Nano ZnO的抗氧化性能和抑菌性能。结果表明,以藤茶黄酮为生物模板制备的Nano ZnO粒子为六方晶系纤锌矿结构,结晶度良好,晶粒平均尺寸约为32.7nm;该纳米材料具有良好的抗氧化活性,2.5g/L的Nano ZnO溶液对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)的清除率为98.24%;藤茶黄酮绿色合成Nano ZnO对金色葡萄球菌的抑菌活性高于传统化学合成的Nano ZnO。绿色合成Nano ZnO的最小抑菌浓度为1.00 g/L,而化学合成Nano ZnO的最小抑菌浓度为1.50 g/L。

ZnO nanoparticles(Nano ZnO) were synthesized via solution combustion method with flavone from Ampelopsis grossedentata as raw materials and characterized by FTIR, XRD, SEM and EDS. The antioxidant and bacteriostatic properties of the greenly synthesized Nano ZnO were evaluated by Brand-Williams method, inhibition zone method and minimum inhibitory concentration method. The results indicated that the greenly synthesized Nano ZnO had a hexagonal wurtzite structure with good crystallinity and its average grain size was about 32.7 nm. Moreover, the Nano ZnO had good antioxidant activity. When the mass concentration of Nano ZnO was 2.5 g/L, the scavenging rate of 1,1-diphenyl-2-picrylhydrazyl free radical(DPPH·) reached 98.24%. Furthermore, the antibacterial activity of greenly synthesized Nano ZnO against Staphylococcus aureus was higher than that of traditional chemically synthesized Nano Zn O. The minimum bacteriostatic concentration of greenly synthesized Nano Zn O was 1.00 g/L, while that of chemically synthesized Nano ZnO was 1.50 g/L.