凝结芽孢杆菌发酵产L-乳酸的蛋白组学分析

Proteomic Analysis of L-lactic Acid Produced by Bacillus coagulans

采用3种碳源(葡萄糖、玉米淀粉糊化液和玉米淀粉)凝结芽孢杆菌BCS13002同步糖化发酵生产L-乳酸,并通过蛋白组学技术分析不同碳源条件下生产L-乳酸差异的生物学机制。结果表明:在葡萄糖、玉米淀粉糊化液和玉米淀粉3种碳源条件下同步糖化发酵,L-乳酸含量分别为(10.23±0.16)g/L,(11.75±0.20)g/L和(0.26±0.02)g/L,共鉴定出454个差异表达蛋白质,这些蛋白质主要与糖酵解/糖异生和三羧酸循环途径有关,还与HIF-1信号通路、丙酮酸代谢、碳代谢、泛酸和辅酶A生物合成、果糖代谢、双组分调节等途径有关。当玉米淀粉为发酵碳源时,凝结芽孢杆菌BCS13002几乎不能通过糖酵解/糖异生产生L-乳酸。导致L-乳酸产量不同的差异蛋白质主要与糖酵解/糖异生和三羧酸循环途径有关。

The aim of this research was that using technology of simultaneous saccharification and fermentation to ferment three different carbon sources(glucose,corn starch gelatinization solution,corn starch)to produce L-lactic acid and proteomics to analysis the difference of the production under three carbon sources by Bacillus coagulans BCS13002.The results showed that the content of L-lactic acid were(10.23±0.16)g/L,(11.75±0.20)g/L,(0.26±0.02)g/L under the carbon sources of glucose,corn starch gelatinized liquid,corn starch liquid conditions,respectively.Furthermore,454 differential expressed proteins were identified under three different carbon sources and these were mainly involved in the glycolysis/gluconeogenesis and tricarboxylic acid cycle pathways,followed by HIF-1 signaling pathway,pyruvate metabolism,carbon metabolic,pantothenic acid and CoA biosynthesis,fructose metabolism,two-component system.Moreover,the Bacillus coagulans BCS13002 could not produce the L-lactic acid with using corn starch liquid by glycolysis/gluconeogenesis metabolism pathway.Above all the content of L-lactic acid mainly related to the difffernetial proteins in the glycolysis/gluconeogenesis and tricarboxylic acid cycle metabolism.