超声辅助纤维素酶法制备杏鲍菇蛋白质工艺优化

Optimization of Protein Preparation of Pleurotus eryngii by Ultrasonic Assisted Cellulase

为提高杏鲍菇蛋白提取率,采用超声波破碎辅助纤维素酶法水解杏鲍菇子实体,制备杏鲍菇蛋白。在单因素试验的基础上,选择料液比、超声功率、超声时间和纤维素酶添加量为影响因素,以蛋白质提取率为指标,进行四因素三水平响应面优化,得到杏鲍菇蛋白质最佳提取工艺,确定影响杏鲍菇蛋白提取率的因素分别为:料液比>超声时间>纤维素酶添加量>超声功率,得到最佳提取条件为:料液比1∶30(g/mL)、超声功率400 W、超声时间20 min、纤维素酶添加量2%。实际提取率75.7%,理论提取率75.1%,相对偏差0.79%。

In order to improve the protein extraction rate of Pleurotus eryngii,the seedlings of Pleurotus eryngii were prepared by ultrasonic disruption and cellulase hydrolysis.On the basis of single factor experiment,the ratio of material to liquid,ultrasonic power,ultrasonic time and cellulase was selected as the influencing factors,and the protein extraction rate was used to optimize the four factors and three levels of response surface to obtain the most protein of Pleurotus eryngii.The optimum extraction factors for determining the extraction rate of Pleurotus eryngii were:material-liquid ratio>ultrasonic time>cellulase addition amount>ultrasonic power,and the best extraction conditions were:ratio of material to liquid 1∶30(g/mL),the ultrasonic power was 400 W,the ultrasonic time was 20 min,and the cellulase addition amount was 2%.The actual extraction rate was 75.7%,and the theoretical extraction rate was 75.1%,and the relative deviation was 0.79%.