近红外免疫层析技术快速检测贝类食品中食源性A群轮状病毒

Rapid detection of foodborne group A rotavirus in shellfish foods based on near-infrared immunochromatography

目的建立近红外免疫层析技术快速、灵敏的检测贝类食品中食源性A群轮状病毒的方法。方法采用近红外荧光标记轮状病毒单克隆抗体4E1,研制A群轮状病毒的近红外免疫层析试纸条及配套标准物质,用近红外荧光扫描仪检测。结果本方法对A群轮状病毒近红外免疫荧光法最低检测限为0.5μg/mL,在45min内即可完成检测,与肠道EV71病毒、腺病毒、诺如病毒、甲肝病毒均无交叉反应。通过效果评价试验发现,与传统检测方法、普通聚合酶链式反应及胶体金法相比较,本文所建立贝类食品中轮状病毒的近红外荧光法检测时间最短,检测限最低。结论本方法对检测贝类食品中食源性A群轮状病毒具有良好的特异性和较高的灵敏度。

Objective To establish a fast and sensitive method for detecting foodborne group A rotavirus in shellfish by near infrared immunochromatography.Methods The monoclonal antibody 4 E1 of rotavirus was labeled with near-infrared fluorescence.Near-infrared immunochromatographic strips and related standard substances of group A rotavirus were developed and detected by near-infrared fluorescence scanner.Results Near infrared fluorescence was completed in 45 min,the detection limit was 0.5μg/mL,and no cross reaction with EV71 virus,adenovirus,nor virus and hepatitis A virus.Evaluation by the test found,the detection time of near-infrared fluorescence of rotavirus was the shortest and the detection limit was the lowest compared with conventional methods,PCR and colloidal gold.Conclusion NIR immunoassay for the detection of rotavirus in shellfish foods has good specificity and high sensitivity.