光石韦中芒果苷和异芒果苷的一测多评含量测定方法的建立

Establishment of a method for the determination of mangiferin and ismangiferin in Pyrrosiacalvata(Bak.)Ching by quantitative analysis of multi-components by single marker

目的建立光石韦中芒果苷和异芒果苷的一测多评含量测定方法。方法采用高效液相色谱法(high performance liquid chromatography,HPLC),十八烷基硅烷键合硅胶为固定相的色谱柱(4.6 mm×250 mm,5μm),乙腈-0.1%甲酸(12:88,V:V)为流动相;检测波长为256 nm。流速1.0 mL/min;柱温30℃,相对校正因子0.894,相对保留时间1.18 s。结果对9批光石韦药材含量测定,分别用外标法和一测多评法计算芒果苷和异芒果苷含量,结果两种方法计算结果差异极小。结论该一测多评法准确、简便可用于光石韦中芒果苷和异芒果苷的含量测定。

Objective To establish a method for the determination of mangiferin and ismangiferin in Pyrrosiacalvata(Bak.)Ching.by quantitative analysis of multi-components by single marker.Methods High performance liquid chromatography(HPLC)was used with acetonitrile-0.1%formic acid(12:88,V:V)as mobile phase in a column(4.6 mm×250 mm,5μm)and octylsilane bonded silica gel as stationary phase.The detection wavelength was 256 nm.The flow rate was 1.0 mL/min.The column temperature was 30℃,the relative correction factor was 0.894,and the relative retention time was 1.18 s.Results For the determination of the contents of 9 batches of Pyrrosiacalvata(Bak.)Ching,the contents of mangiferin and ismangiferin were calculated by using external standard method and quantitative analysis of multi-components by single marker method respectively.Conclusion The method is accurate and simple for the determination of mangiferin and ismangiferin in Pyrrosiacalvata(Bak.)Ching.