响应面法优化稷山板枣枣皮红色素的提取工艺

Optimization of extraction process of red pigment from Jishan jujube peel by response surface method

以稷山板枣为原料,响应面优化碱法提取枣皮红色素。在单因素实验基础上,选取三因素三水平Box-Behnken实验设计,以色素提取液吸光度值为响应值,考察NaOH浓度、提取时间和提取温度对色素提取效果的影响。结果表明,最优工艺条件为:NaOH浓度0.2mol/L,提取时间2.3h,提取温度71℃,此时吸光度值为0.545,与模型预测值0.558接近,说明该模型能较好的预测实验结果。各因素对提取效果影响的大小顺序为NaOH浓度>提取时间>提取温度。本研究用响应面法优化了枣皮红色素的提取工艺,为板枣的深加工提供了一定的理论基础。

Using Jishan Jujube as raw material,red pigment was extracted by response surface method in alkaline conditions. Based on the single factor experiments,a three-factor and three-level experiment was developed by boxbehnken design. The absorbance value of pigment extraction solution was selected as the response value to investigate effects of NaOH concentration,extraction time and extraction temperature. The results showed that the optimal process conditions were as follows :NaOH concentration 0.2 mol/L,extraction time 2.3 h,and extraction temperature 71℃. Under the optimum extraction conditions,the absorbance value was 0.545,which was close to the predicted value of 0.558,indicating that the model could well predict experimental results. The order of influence of each factor on the extraction effect was NaOH concentration > extraction time > extraction temperature. The study optimized the pigment extraction process and provided a theoretical basis for further study of jujube process.