玫瑰纯露提取工艺优化及其抑菌和抗氧化活性研究

Study on Extraction Process Optimization of Rose Hydrosol and Its Antibacterial and Antioxidant Activities

以"中天"玫瑰花朵为试材,采用水蒸气蒸馏法提取玫瑰纯露,通过响应面试验优化了玫瑰纯露的提取工艺,并评价了玫瑰纯露的抑菌和抗氧化活性,以期为玫瑰纯露的开发利用提供参考依据。结果表明:在花液比为1∶1.7 g·mL-1、回流时间为7 min、蒸馏温度为120℃的条件下,获得的纯露感官评分最高;在该条件下提取的玫瑰纯露对DPPH·和超氧阴离子的清除率分别为24.66%和5.03%,对微生物生长具有明显抑制作用,表明提取的玫瑰纯露具有较好的抑菌和抗氧化活性。

Taking rose ‘Zhongtian’ petals as materials,hydrosols were extracted by using the method of steam distillation.The extraction process of rose hydrosol was optimized by response surface methodology,and antibacterial and antioxidant activities of rose hydrosol were evaluated,in order to provide basis for the development and utilization of rose hydrosol.The results showed that under the condition of flower-liquid ratio of 1∶1.7 g·mL-1,reflux time of 7 minutes and distillation temperature at 120 ℃,the obtained hydrosol showed the highest sensory score.Under this condition,the scavenging rate of rose hydrosol against DPPH· and superoxide anion were 24.66% and 5.03%,respectively,and which could obviously inhibit the growth of microorganisms,suggesting that the extracted rose hydrosol had strongly antibacterial and antioxidative activities.