宁夏地产黄芪药材质量评价研究

Study on the Quality Evaluation of Ningxia Astragali Radix

以宁夏栽培黄芪药材为试材,采用HPLC法测定不同产区的二年生和三年生黄芪药材中黄芪甲苷和毛蕊异黄酮葡萄糖苷的含量;建立宁夏地产黄芪药材HPLC-ELSD-DAD对照指纹图谱,采用相似度评价对指纹图谱进行分析;运用主成分分析进行模式识别研究,以期为科学评价及有效控制宁夏地产黄芪药材质量提供参考依据。结果表明:HPLC-DAD-ELSD指纹图谱有31批黄芪药材与生成的对照指纹图谱相似度分别在0.90和0.85以上;黄芪生长年限越长,其第一主成分的累积贡献率越高;宁夏盐池产区黄芪药材与其它产区有明显差别。综上所述,建立的HPLC-ELSD-DAD指纹图谱特征性良好,为全面控制宁夏地产黄芪药材质量提供了参考依据。

Cultivated Astragali Radix in Ningxia was used as experimental material,and determined the contents of astragaloside and isoflavone glucoside in two years old and three years old Astragali Radix from different producing areas by HPLC.Established HPLC-ELSD-DAD contrast fingerprint of Astragali Radix in Ningxia,analyzed fingerprint by similarity evaluation.Researched on pattern recognition by principal component analysis,in order to provide basis for scientific evaluation and effective control of the quality of Astragali Radix in Ningxia.The results showed that the similarity between HPLC-ELSD-DAD fingerprint of 31 batches Astragali Radix and reference fingerprint was above 0.90 and 0.85.The longer growth years of Astragali Radix,the higher cumulative contribution rate of the first principal component.The main components content of Astragali Radix in Yanchi area of Ningxia were obviously different from that of other producing areas.In conclusion,the established HPLC-ELSD-DAD fingerprint had good characteristics,which provided a reference for the overall quality control of Astragali Radix in Ningxia.