hsa-miR-10a-5p在重症社区获得性肺炎中的应用研究

Application of hsa-miR-10a-5p in severe community acquired pneumonia

目的探究hsa-miR-10a-5p在重症社区获得性肺炎(SCAP)中的作用,评估hsa-miR-10a-5p作为SCAP标志物的潜能。方法利用microRNA测序(27个SCAP血样和10个健康对照血样)和生物信息学方法探究hsa-miR-10a-5p指示社区获得性肺炎严重性的能力。结合mRNA测序(41个SCAP血样和10个健康对照血样)和靶基因预测及基因功能富集分析来探究hsa-miR-10a-5p在SCAP中的作用。结果hsa-miR-10a-5p水平在SCAP中显著下调,且死亡组下调更多。hsa-miR-10a-5p水平与急性生理功能和慢性健康状况评分系统Ⅱ(APACHEⅡ)评分呈负相关,且microRNA水平预测SCAP预后的受试者工作特征曲线下面积(AUC)为0.776。SCAP发生时,hsa-miR-10a-5p预测靶向的基因能调控与肺炎发展密切相关的生物学过程和通路。结论hsa-miR-10a-5p能提示社区获得性肺炎严重性,且能预测预后。hsa-miR-10a-5p可作为SCAP的潜在标志物。

Objective To explore the role of hsa-miR-10a-5p in severe community acquired pneumonia(SCAP)and to evaluate the potential of hsa-miR-10a-5p as a SCAP biomarker.Methods microRNA sequencing(27 SCAP blood samples and 10 healthy control blood samples)and bioinformatics methods were used to explore hsa-miR-10a-5p as a potential marker of the severity of community acquired pneumonia.The role of hsa-miR-10a-5p in SCAP was examined in combination with mRNA sequencing(41 SCAP blood samples and 10 healthy control blood samples),target gene prediction,and gene functional enrichment analysis.Results hsa-miR-10a-5p level was significantly down-regulated in SCAP,with more down-regulation in the death group.The level of hsa-miR-10a-5p was negatively correlated with the acute physiology and chronic health evaluationⅡ(APACHEⅡ)score,and the area under the receiver operating characteristic curve(AUC)of the microRNA level predicting SCAP prognosis was 0.776.When SCAP occurred,the predicted targeted gene of hsa-miR-10a-5p regulated biological processes and pathways whick were closely related to the development of pneumonia.Conclusions hsa-miR-10a-5p can indicate the severity of community acquired pneumonia and predict the prognosis as a potential biomarker.