趋化因子受体基因多态性与妇女宫颈癌前病变的相关性分析

Correlation between CCR gene polymorphism and cervical precancerous lesions in women

目的观察趋化因子受体(CCR)2-64I(rs1799864)和CCR5-Δ32(rs333)多态性与宫颈癌前病变的相关性。方法研究对象包括35例宫颈癌前病变患者;35例非宫颈癌前病变妇女。采用聚合酶链反应-限制性片段长度多态性检测所有受试者CCR2-64I和CCR5-Δ32多态性。结果携带CCR2-64I纯合子或杂合子基因型者宫颈癌前病变风险较低(OR=0.38,P=0.007)。CCR2-64I A等位基因携带者宫颈癌前病变风险也较低(OR=0.35,P=0.002),但与CCR5-Δ32多态性无相关性(P>0.05)。结论研究表明,CCR 2-64I(rs1799864)多态性携带者宫颈癌前病变风险低且不易感染HPV16。

Objective To investigate the correlation between chemokine receptors(CCR)2-64I(rs1799864),CCR5-Δ32(rs333)polymorphisms and susceptibility to cervical precancerous lesion in women.Methods Included in this study were 35 women with cervical precancerous lesions and another 35 without cervical precancerous lesions.Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)analysis was used to determine the polymorphisms at CCR2-64I and CCR5-Δ32.Results The individuals carrying heterozygous or homozygous genotypes(GA+AA)for CCR2-64I polymorphisms seemed to be at lower risk for cervical precancerous lesion(OR=0.38,P=0.007).The same was observed for the carriers of CCR2-64I allele A(OR=0.35,P=0.002),while no association was detected(P>0.05)with CCR5-Δ32 polymorphism.Conclusion Our study showed a protective effect of CCR2-64I(rs1799864)polymorphism against cervical precancerous lesions and susceptibility to HPV type 16 infection.