两种方法制备纳米金原位增敏金标免疫层析试验的比较

Comparison of two methods for preparing gold nanoparticles in situ sensitization gold label immunochromatographic test

目的比较两种化学方法制备纳米金的增敏效果,找出相对合适的制备纳米金增敏金标免疫层析试验的方法。方法以金标免疫层析试验检测血浆绒毛膜促性腺激素(HCG)为基础,用盐酸羟胺和抗坏血酸还原氯金酸制备纳米金颗粒,判断纳米金原位所形成的纳米颗粒颜色,检测其灵敏度。结果盐酸羟胺还原法增敏血浆HCG金标免疫层析试验,相对增敏前检测灵敏度提高约16倍,且重复性较好。抗坏血酸还原法增敏血浆HCG金标免疫层析试验,相对增敏前检测灵敏度提高约4倍。结论盐酸羟胺还原法制备纳米金对血浆HCG金标免疫层析试验增敏倍数较高,重复性较好,试验结果可靠,可推广使用。

Objective To compare the sensitizing effects of two chemical methods for preparing gold nanoparticles,and to find a relatively suitable method for preparing gold nanoparticles to enhance the sensitization gold label immunochromatographic test.Methods Based on the gold-labeled immunochromatographic test to detect plasma chorionic gonadotropin(HCG),gold nanoparticles were prepared by reducing chloroauric acid with hydroxylamine hydrochloride and ascorbic acid,and the color of nanoparticles formed by gold nanoparticles in situ was determined and its sensitivity was detected.Results Hydroxylamine hydrochloride reduction method sensitized the plasma HCG gold-labeled immunochromatographic test.The detection sensitivity was about 16 times higher than that before sensitization,and the repeatability was good.Ascorbic acid reduction method sensitized the plasma HCG gold-labeled immunochromatographic test,and the detection sensitivity was about 4 times higher than that before sensitization.Conclusion Hydroxylamine hydrochloride reduction method which prepare gold nanoparticles has a higher sensitivity to plasma HCG gold-labeled immunochromatographic test,good repeatability,and reliable test results,which can be widely used.