花椒麻味素含量两种检测方法之比较

Comparison of Two Methods for Determination of Numbing Components Content in Zanthoxylum bungeanum

为确定花椒麻味素含量快速测定方法,利用华州黄盖、韩城大红袍、华州大红袍花椒及其无水乙醇提取的花椒麻味素,对甲醛滴定法(A)和凯氏定氮法(B)检测法进行了实验比较研究。结果表明,花椒麻味素含量(y)华州黄盖>韩城大红袍>华州大红袍;花椒乙醇提取物A法麻味素含量平均值()是B法麻味素含量平均值()的19.56倍,用19.56将x校正为麻味素含量y′后,t测验说明y′与差异显著,用模型y′=2.3839+8.8147将x校正为麻味素含量y′,则y′与间无差异;用B法测定花椒粉末样N含量x′,用模型y″=0.0151x′2-1.0216 x′+26.8190将x′校正为麻味素含量y″,t测验说明y与y″无显著差异。研究结果表明,定氮仪法测定花椒N含量,并建立麻味素含量与N含量关联模型,再将N含量转换为麻味素含量,可实现用凯氏定氮法间接检测花椒麻味素含量。

To establish a rapid determination method of the numbing components content of Zanthoxylum bungeanum,it can be used to evaluate the quality and its products.Therefore,the formaldehyde method(A)was used to titrate the numbing components content Y,Kjeldahl method(B)determination of N content X.The test results showed that Y value follows an order:Huazhou Huanggai>Hancheng Dahongpao>Huazhou Dahongpao.The value of ethanol extract of prickly ash A was 19.56 times of the value of B,19.56 was used to calibrate the X to the numbing components content Y′.T test showed a significant difference between Y and.By substituting into Y′=2.3839+8.8147,the value of numbing components content Y′was obtained.The test showed no difference between Y′and.The content X′of N in prickly ash powder was determined by B method.By substituting X′into Y″=0.0151X′2-1.0216X′+26.8190,the corrected value of numbing components content Y″was obtained.T test showed that there was no significant difference between Y and Y″.The above conclusion showed that after determining the N content of Zanthoxylum bungeanum by Kjeldahl method,the correlation model between numbing components content and N content was established,the N content is then converted to the numbing components content.The numbing components content in Zanthoxylum bungeanum can be determined by Kjeldahl method.