MEAM1烟粉虱隐种对3种新烟碱类杀虫剂交互抗性及交互现实遗传力的分析

Cross-resistance and Realized Heritability Analysis of Whitefly Bemisia tabaci MEAM1 cryptic Species to Three Neonicotinoids

【目的】评估MEAM1烟粉虱隐种[Bemisia tabaci(Gennadius)]对3种新烟碱类杀虫剂的交互抗性风险,预测交互抗性发展速率,为科学合理用药提供依据。【方法】采用群体汰选法获得抗性品系,采用数量遗传学的域性状分析法估算交互抗性现实遗传力(h2),并预测不同选择压下交互抗性发展速率。【结果】在50%~70%药剂选择压力下,MEAM1烟粉虱隐种连续汰选9代后,吡虫啉抗性汰选品系对噻虫嗪和啶虫脒交互抗性倍数分别上升了8.72和19.21倍,分别达到低等和中等交互抗性水平,平均交互现实遗传力h2分别为0.1981和0.2854;啶虫脒抗性汰选品系对吡虫啉和噻虫嗪交互抗性倍数分别上升了10.08和9.83倍,表现出中等和低等交互抗性水平,平均交互现实遗传力h2分别为0.2423和0.1281;噻虫嗪抗性汰选品系对吡虫啉和啶虫脒交互抗性倍数分别上升了3.12和3.11倍,均处于敏感性下降阶段,平均交互现实遗传力h2分别为0.1421和0.0680。【结论】吡虫啉抗性汰选品系对噻虫嗪的交互抗性风险小于对啶虫脒的交互抗性风险;啶虫脒抗性汰选品系对噻虫嗪的交互抗性风险小于对吡虫啉的交互抗性风险;噻虫嗪抗性汰选品系对啶虫脒的交互抗性风险小于对吡虫啉的交互抗性风险。

【Objective】To assess the cross-resistance risk of whitefly Bemisia tabaci(Gennadius)MEAM1 cryptic species to three neonicotinoid insecticides and predict the rate of cross-resistance development in the hope of providing the reference for scientific and rational application of insecticides.MEAM1[Bemisia tabaci(Gennadius)]【Methods】The cross-resistance strain was developed by group selection,and the realized heritability(h2)of Bemisia tabaci cross-resistance was estimated by Tabashnik's method.【Results】Results showed that after being selected by imidacloprid,thiamethoxam and acetamiprid for 9 generations under 50%-70%selection pressure,the cross-resistance ratio of MEAM1 cryptic species to the three neonicotinoids differed from each resistance selected strain.The cross-resistance ratio of imidacloprid selected strain to thiamethoxam and acetamiprid increased by 8.72-fold and 19.21-fold,respectively,which were low and medium cross-resistance levels and their interactive realized heritability h2 were 0.198,1 and 0.285,4,respectively.The cross resistance ratio of acetamiprid strain to imidacloprid and thiamethoxam increased by 10.08-fold and 9.83-fold,respectively,which were low and medium cross-resistance levels and the interactive realized heritability h2 were 0.242,3 and 0.128,1,respectively.The cross-resistance ratio of thiamethoxam selected strain to imidacloprid and acetamiprid increased by 3.12-fold and 3.11-fold,respectively,which were in the sensitivity decrease stage and the cross realized heritability h2 was 0.142,1 and 0.068,0,respectively.【Conclusion】The risk of the imidacloprid selected strain developing cross-resistance to thiamethoxam was less than that of acetamiprid.The risk of the acetamiprid selected strain developing cross-resistance to thiamethoxam was less than that of imidacloprid.The risk of the thiamethoxam selected strain developing cross-resistance to acetamiprid was less than that of imidacloprid.