高效液相色谱法测定酱油中苯甲酸的不确定度评定

Uncertainty Evaluation for the Determination of Benzoic Acid in Soy Sauce by High Performance Liquid Chromatography

采用高效液相色谱法(HPLC)评定酱油中苯甲酸的不确定度。根据JJF 1135-2005《化学分析测量不确定度评定》和JJF 1059.1-2012《测量不确定度评定与表示》,建立高效液相色谱法测定苯甲酸不确定度测量的数学模型,分析不确定度来源。通过对不确定度来源(测量重复性、天平重复性误差及示值误差、容量瓶允许差、温度效应、标准品、移液器误差、标准系列拟合、回收率和液相色谱仪)进行量化和合成。得出酱油中苯甲酸含量为(0.625±0.025)g/kg(k=2)。影响结果的主要因素为标准系列溶液配制、回收率和标准曲线拟合。本研究为酱油中苯甲酸的能力验证以及向社会出具官方检测报告提供科学、准确的根据。

The uncertainty of benzoic acid in soy sauce was evaluated by high performance liquid chromatography(HPLC).According to JJF 1135-2005 Evaluation of Uncertainty in Chemical Analysis Measurement and JJF 1059.1-2012 Evaluation and Expression of Uncertainty in Measurement,a mathematical model was established for uncertainty evaluation.The uncertainty sources(measurement repeatability,repeatability error and the error value for balance,volumetric flask allowed difference,temperature effect,standard,pipetting error,standard series fitting,recovery rate and liquid chromatograph)were quantified and synthesized.It was concluded that content of benzoic acid in soy sauce was(0.625±0.025)g/kg(k=2).The evaluation results showed that the main factors influencing the results were the preparation of standard series solution,the recovery rate and the fitting of standard curve.This study provides a scientific and accurate basis for the ability verification of benzoic acid in soy sauce and the issuance of official test reports to the society.