辣蓼散不同极性段提取物体外抑菌效果研究

Study on antibacterial effect of the extracts from different polar of Laliao decotion in vitro

试验研究辣蓼散不同极性段提取物对兽医临床常见致病菌的体外抑菌效果。采用水和乙醇等不同极性溶剂对辣蓼散进行提取,用纸片法测定6种提取物对绿脓杆菌、鸡白痢沙门氏菌、鸡大肠杆菌等5种病原菌的体外抑菌效果,并用试管二倍稀释法测定各提取物的最小抑菌浓度(MIC)。结果表明:辣蓼散不同极性段提取物对5种病原菌有不同的抑制作用;辣蓼散水煎醇提物的抑菌效果最为明显,对鸡白痢沙门氏菌、鸡大肠杆菌以及金黄色葡萄球菌均有较好的抑菌效果,MIC均为15.62 g/L,对白色念珠菌与绿脓杆菌的MIC均为31.25 g/L。研究结果为辣蓼散在畜牧业中的应用提供参考。

To study the antibacterial effect of different polar extracts of Laliao decotion on common bacteria in veterinary medicine in vitro, water and different concentrations of alcohol were used to extract different dissolvent of Laliao decotion. The in vitro antibacterial activity of the extracts against five pathogenic bacteria, including Pseudomonas aeruginosa, Salmonella pullorum, chicken Escherichia coli, Staphylococcus aureus and Candida albicans, was determined by disc diffusion test. The minimum inhibitory concentration(MIC) of each extract was determined by tube double dilution method. The results showed that the extracts of different polar of Laliao decotion had different inhibitory effects on five pathogenic bacteria. The antibacterial effect of the water-alcohol extract was most obvious. It had good antibacterial effect on Salmonella pullorum, Escherichia coli and Staphylococcus aureus, and the MIC was 15.62 g/L. The MIC of both Candida albicans and Pseudomonas aeruginosa was 31.25 g/L. The research results provide references for the application of Laliao decotion in animal husbandry.