烟碱偏好模型不同阶段大鼠海马组织中23种细胞因子水平的变化

Variations of 23 cytokine levels in rat hippocampus at different stages of a nicotine preference model

为了探究烟碱对大鼠海马组织中炎症相关细胞因子表达的影响,利用条件位置偏好(Conditioned place preference,CPP)装置,分别以0.2和0.6 mg/kg烟碱剂量成功构建大鼠烟碱CPP获得、消退和重建模型,应用悬液芯片技术对大鼠海马组织中23种炎症相关的细胞因子水平进行同时检测。通过单因素方差分析探讨了烟碱偏好不同阶段大鼠海马组织中表达水平有显著差异的细胞因子。结果表明:①低和较高剂量下,烟碱能够促进细胞因子白细胞介素(Interleukin,IL)-1α和IL-10的表达,抑制IL-6、IL-18、生长相关癌基因/角细胞趋化因子(Growth-related oncogene/keratinocyte chemoattractant,GRO/KC)以及受激活调节正常T细胞表达和分泌因子(Regulated upon activation normal T cell expressed and secreted factor,RANTES)的表达;②大鼠海马中GRO/KC水平易受烟碱影响,单一烟碱作用会降低GRO/KC的表达,在获得-消退阶段,该降低作用减弱,在消退-重建阶段该降低作用再次恢复至获得阶段,该细胞因子可能参与烟碱的潜在抗炎作用机制;③其他5种细胞因子表达量的变化趋势与GRO/KC不相同甚至相反,有必要在接下来的研究中进一步通过动物炎症模型探究烟碱对炎症影响的作用机制。

To investigate the effects of nicotine on the expression of inflammation-related cytokines in the hippocampus of rats,a conditional place preference(CPP)device was used to establish the acquisition,extinction and reinstatement of nicotine CPP in rats at the nicotine doses of 0.2 and 0.6 mg/kg.A total of 23 inflammation-related cytokines were detected simultaneously in the hippocampus at different stages(acquisition,extinction and reinstatement)by the suspension chip technique.The one-way variance analysis was adopted to analyze the differential cytokine expressions in the hippocampus at different stages using a nicotine preference model.The results showed that:1)Nicotine promoted the expressions of interleukin(IL)-1αand IL-10 and inhibited the expressions of IL-6,IL-18,growth-related oncogene/keratinocyte chemoattractant(GRO/KC)and regulated upon activation normal T cell expressed and secreted factor(RANTES)at low and higher doses.2)The GRO/KC level in rat hippocampus was susceptible to nicotine,which reduced GRO/KC expression,while the reduction effect was weakened during the acquisition-extinction stage and was restored to the level at the acquisition stage during the extinction-reinstatement stage,the evidence therefore suggested that GRO/KC might be involved in the potential anti-inflammatory mechanism of nicotine.3)The variation trends of the other five cytokine levels were different or even contrary to GRO/KC.Further studies are needed to understand the inflammatory mechanism of nicotine through the rat and other animal inflammation models.