烟碱对映异构体手性分离方法研究进展

Progress on analytical chiral separation of nicotine enantiomers

烟碱对映异构体[S-(-)-烟碱和R-(+)-烟碱]的手性分离是研究其药理学差异的基础,是近几年的研究热点。综述了近40年烟碱对映异构体的手性分离方法,如液相色谱法、超临界流体色谱法、气相色谱法、毛细管电泳法和核磁共振法等,对各方法进行了讨论,并对该领域的发展前景作了展望。其中,基于改性多糖类及大环抗生素类手性固定相的液相色谱法和超临界流体色谱法具有快速、高效、灵敏和环保等优势,在烟碱手性分离中应用最为广泛。

The chiral separation of nicotine enantiomers[S-(-)-nicotine and R-(+)-nicotine]is one of current research focuses and a key step on stereoselective pharmacokinetics study of nicotine.The research progress on chiral separation methods of nicotine enantiomers in recent 40 years was reviewed and discussed,including liquid chromatography,supercritical fluid chromatography,gas chromatography,capillary electrophoresis,nuclear magnetic resonance methods,etc.The development in this field was prospected,wherein liquid chromatography and supercritical fluid chromatography containing chiral stationary phases applied with modified polysaccharides and macrocyclic antibiotics were most widely used due to their advantages being fast,efficient,sensitive and environmentally friendly.