基于废蔗渣为原料的小球藻产微生物油脂的研究

Study on microbial oil producted by chlorella based on waste bagasse

基于废蔗渣为原料,通过将蔗渣中的各类含碳有机物水解为可供小球藻吸收利用的糖类,对比两种水解方式获取更高糖浓度的蔗渣水解液,用此类方法得到的蔗渣水解液异养培养小球藻。通过测定分析小球藻的生长量、产油量、产油效率三项指标获取能有效培养小球藻产油的水解液含量。在此基础上,通过改变小球藻不同的生长条件,得到最适宜其产油的条件。结果表明:高温灭菌能增加水解液含糖量;水解液体积为到3mL即总糖浓度为0.7200g/L时小球藻各项指标达到最大化;p H的范围对小球藻的产油影响最大,小球藻最佳产油条件为尿素含量100mmol/L,光照时长18h,p H为7。本实验方法便捷,成本低廉,具有较好的工业化应用前景,为固废回收利用及微藻产油经济化提供了参考。

Based on waste bagasse as raw material,various types of carbon-containing organic matter in bagasse were hydrolyzed into sugars that can be absorbed and used by chlorella,and two kinds of hydrolysis methods were used to obtain a bagasse hydrolysate with a higher sugar concentration.Heterotrophic culture of chlorella with bagasse hydrolysate.The content of the hydrolysate which can effectively cultivate the oil production of chlorella was obtained by measuring and analyzing the three indexes of growth,oil production and oil production efficiency of chlorella.On this basis,by changing different growth conditions of chlorella,the optimal conditions for its oil production were obtained.The results show that:high temperature sterilization can increase the sugar content of the hydrolysate;when the volume of the hydrolysate is 3 m L,that is,the total sugar concentration is 0.7200 g/L,the indicators of chlorella are maximized;The effect is greatest.The optimal oil production conditions for chlorella are 100 mmol/L urea,18 h light duration,and p H 7.This experimental method is convenient,low cost,and has good industrial application prospects,which provides a reference for the solid waste recycling and economical production of microalgae oil.