A Network Pharmacology Analysis of Cang Fu Dao Tan Formula for the Treatment of Obese Polycystic Ovary Syndrome

苍附导痰汤治疗肥胖型多囊卵巢综合征的网络药理学研究

Objective To explore the potential molecular targets of Cang Fu Dao Tan Formula(CFDTF)in the treatment of obese polycystic ovary syndrome(PCOS)using network pharmacology and bioinformatic approaches.Methods The potential blood-entry active compounds and targets of CFDTF were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),and obese PCOS related gene targets were retrieved from GeneCard databases.A protein-protein interaction(PPI)network of CFDTF component-targets and obese PCOS diseasetargets was constructed using STRING.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis of the intersection network were conducted using a Bioconductor database.Results In total,114 active compounds were screened according to oral bioavailability(OB)and drug similarity(DL),and 328 targets related to these constituents were obtained.Further,2559 target genes directly related to obese PCOS were obtained from the GeneCard databases,and 82 genes were obtained from the intersection of the component-target and disease-target PPI network.These genes were mainly involved in response to steroid hormones,nutrient levels and lipopolysaccharide as well as in other biological processes.Their molecular functions were mainly related to nuclear receptor activity,phosphatase binding and cytokine activity,and they were enriched in the cytoplasm,cell membrane and membrane region.Conclusions CFDTF consists of 114 active compounds that are responsible for its pharmacodynamic effects.It mainly regulates the AGE-RAGE signaling pathway in diabetic complications,bladder cancer,and hepatitis B and in other signaling networks.These findings provide certain theoretical and scientific basis for the treatment of obese PCOS with Chinese medicine.

目的应用网络药理学和生物信息学方法,探讨苍附导痰汤干预肥胖型多囊卵巢综合征的作用机制。方法从中药系统药理学数据库和分析平台(TCMSP)中检索苍附导痰汤的活性成分和靶点,从GeneCard数据库中检索肥胖型多囊卵巢综合征的靶点,利用STRING软件构建苍附导痰汤成分靶点与疾病靶点交集的蛋白质相互作用(PPI)网络,利用Bioconductor数据库进行GO和KEGG富集分析。结果根据口服生物利用度(OB)和药物相似性(DL),共筛选出114种活性成分及328个与活性成分相关的靶点,从GeneCard数据库中获得2559个与肥胖型多囊卵巢综合征相关的靶点,将两部分交集所得的82个基因构建PPI网络。这些基因主要与甾体激素、营养水平、脂多糖等生物过程有关;它们的分子功能主要与核受体活性、磷酸酶结合和细胞因子活性有关,并主要富集在细胞质、细胞膜部位。结论苍附导痰汤中的114种活性成分是其药效的重要基础,它们通过调节AGE-RAGE、膀胱癌、乙型肝炎信号通路发挥效应,以上发现为苍附导痰汤治疗肥胖型多囊卵巢综合征提供了一定理论依据。