乳腺癌癌组织中VWCE、MEKK3的表达水平与临床病理、预后的关系分析

Analysis of the relationship between the expression levels of VWCE and MEKK3 in breast cancer tissues and clinicopathology and prognosis

目的研究乳腺癌癌组织中结构域蛋白(VWCE)、有丝分裂原活化蛋白激酶激酶激酶(MEKK3)的表达水平与临床病理、预后的关系。方法选取2015年1月至2016年1月于本院接受治疗的乳腺癌患者130例,分别检测患者癌组织及癌旁组织中VWCE、MEKK3的表达水平;根据患者VWCE/GADPH灰度值>2为高表达,VWCE/GADPH灰度值≦2为低表达将患者分为VWCE高表达组和VWCE低表达组,根据MEKK3/GADPH灰度值>2为高表达,MEKK3/GADPH灰度值≦2为低表达将患者分为MEKK3高表达组和MEKK3低表达组,分析腺癌癌组织中VWCE、MEKK3的表达水平与病理特征关系;分析VWCE、MEKK3的表达水平与患者生存率的关系。结果根据患者VWCE表达和MEKK3的表达将患者分为VWCE高表达组75例、VWCE低表达组55例以及MEKK3高表达组70例、MEKK3低表达组60例;癌组织中VWCE相对表达水平显著低于癌旁组织,差异具有统计学意义(P<0.05);癌组织中MEKK3相对表达水平显著高于癌旁组织,差异具有统计学意义(P<0.05);MEKK3和VWCE的表达水平与患者年龄、病理类型、雌激素受体、孕激素受体无明显的相关性(P>0.05);VWCE低表达表达与肿瘤直径、临床分期和淋巴结有明显的相关性(P<0.05);MEKK3高表达与肿瘤直径、临床分期和淋巴结是否转移有明显的相关性(P<0.05);VWCE高表达组3年生存率为85.33%,显著高于VWCE低表达组的61.82%,差异具有统计学意义(P<0.05);MEKK3高表达组3年生存率为68.57%,显著低于MEKK3低表达组的88.33%,差异具有统计学意义(P<0.05)。结论乳腺癌组织中VWCE呈低表达,MEKK3呈高表达;VWCE的高表达和MEKK3的低表达与乳腺癌患者的肿瘤直径、临床分期、淋巴结是否转移及预后有明显的相关性。

Objective To study the relationship between the expression levels of von Willebrand factor C and epidermal growth factor domains(VWCE)and mitogen-activated protein kinase/extracellular signalregulated kinase kinase kinase 3(MEKK3)in breast cancer tissues and clinicopathology,prognosis. Methods A total of 130 breast cancer patients who were treated in our hospital from January 2015 to January 2016 were selected to detect the expression levels of VWCE and MEKK3 in the cancer tissues and adjacent tissues of the patients. The expression levels of VWCE and MEKK3 in cancer tissues and adjacent tissues were detected.VWCE/GADPH gray value greater than 2 was defined as high expression,while VWCE/GADPH gray value not greater than 2 was defined as low expression. The patients are divided into VWCE high-expression group and VWCE low-expression group. MEKK3/GADPH gray value greater than 2 was defined as high expression,while MEKK3/GADPH gray value not greater than 2 was defined as low expression. The patients are divided into MEKK3 high-expression group and MEKK3-low expression group. The relationship between the expression levels of VWCE and MEKK3 in adenocarcinoma tissues and pathological features was analyzed. The relationship between the expression levels of VWCE and MEKK3 and the survival rate was analyzed. Results According to the expression of VWCE and MEKK3,patients were divided into VWCE high-expression group(75 cases),VWCE low-expression group(55 cases),MEKK3 high-expression group(70 cases)and MEKK3 low-expression group(60 cases). The relative expression level of VWCE in cancer tissue was significantly lower than that in adjacent tissues(P<0.05),while the relative expression level of MEKK3 was significantly higher than that in adjacent tissues(P<0.05). The expression levels of MEKK3 and VWCE were not significantly related to age,pathological types,estrogen receptor or progesterone receptor(P>0.05). The low expression of VWCE and high expression of MEKK3 were related to tumor diameter,clinical stage and lymph node metastasis(P<0.05). The 3-year survival rate in VWCE high-expression group was significantly higher than that in VWCE low-expression group(85.33% vs 61.82%)(P<0.05). The 3-year survival rate in MEKK3 high-expression group was significantly lower than that in MEKK3 low-expression group(68.57% vs 88.33%)(P<0.05). Conclusion Breast cancer tissues have low expression of VWCE and high expression of MEKK3.The high expression of VWCE and the low expression of MEKK3 are significantly related to the tumor diameter,clinical stage,lymph node metastasis and prognosis of breast cancer patients.