连翘的化学成分研究

Study on chemical constituents of Forsythia suspensa

目的对中药连翘Forsythia suspensa(Thunb.)Vhal果实的化学成分进行系统研究。方法将连翘果实用体积分数为95%的乙醇冷浸7 d,用乙酸乙酯萃取,结合各种柱色谱方法对乙酸乙酯部位进行系统地化学成分分离,根据理化性质和光谱数据鉴定化合物的结构。结果分离并鉴定了14个化合物的结构。4个二萜化合物:19-formyl-ent-labda-8(17),13 E-dien-15-oic acid(1);3α,18-dihydroxy-ent-labda-8(17),13 E-dien-15-oic acid(2);3α-hydroxy-ent-labda-8(17),12 E-14-trien-18-ol(3)和3α-hydroxy-ent-labda-8(17),12 E-14-trien-19-oic acid(4)。6个木脂素类:松脂素(5)、松脂素-4-O-β-葡萄糖苷(6)、表松脂酚(7)、连翘脂素(8)、连翘苷(9)和安五脂素(10)。4个三萜类:白桦脂酸(11)、熊果酸(12)、齐墩果酸(13)和methyl-3α-hydroxyolean-18-ene-28-oate(14)。结论化合物2,3,4,10和14均为首次从该植物中分离得到。

Objective To study the chemical constituents of Chinese medicine Forsythia suspensa(Thunb.)Vhal fruits.Methods The fruits of F.suspensa were extracted by using volume fraction 95%ethanol at room temperature for 7 d.The ethanol crude extract was fractionalized by ethyl acetate and followd by using a series of chromatography methods to isolate the compounds.Structures of the isolates were elucidated by their physicochemical properties and spectroscopic data.Results 14 compounds were isolated and identified as 4 diterpenes:19-formyl-ent-labda-8(17),13 E-dien-15-oic acid(1);3α,18-dihydroxy-ent-labda-8(17),13 E-dien-15-oic acid(2);3α-hydroxy-ent-labda-8(17),12 E-14-trien-18-ol(3);3α-hydroxy-ent-labda-8(17),12 E-14-trien-19-oic acid(4).6 lignans:pinoresinol(5);pinoresinol-4-O-β-glucoside(6);epipinoresinol(7);phillygenin(8);phillyrin(9);(+)-anwulignan(10).4 triterpenes:betulinic acid(11);ursolic acid(12);oleanolic acid(13);methyl-3α-hydroxyolean-18-ene-28-oate(14).Conclusion Compounds 2,3,4,10 and 14 were isolated from F.suspense for the first time.