γ-聚谷氨酸的抗氧化及对UV辐射致皮肤细胞光老化的修复作用

Antioxidation of poly-(γ-glutamic acid)and repair of photoagingfibroblasts induced by UV radiation

寻找天然抗氧化物用于修复皮肤光老化问题是目前的研究热点之一。γ-聚谷氨酸(γ-PGA)是由微生物发酵制备的一种天然高分子聚合物材料,具有清除自由基的能力,但其抗氧化、抗皮肤光老化效果及与γ-PGA分子量的关系尚未明确。本文中,笔者考察不同分子量的γ-PGA对超氧阴离子自由基(·O^-2)、羟基自由基(·OH)和1,1-二苯基-2-三硝基苯肼(·DPPH)的清除能力。结果显示:不同分子量的γ-PGA对以上3种自由基均表现出一定程度的自由基清除活性,清除率可分别达31.36%、97.55%和74.64%。通过构建紫外线诱导小鼠皮肤成纤维细胞(L929)氧化损伤光老化模型,确定不同分子量的γ-PGA都能一定程度修复光老化细胞损伤并能减少光老化细胞中氧自由基(ROS)、氮自由基(NO)的增加,其中分子量3.0×10^5的γ-PGA修复光老化细胞损伤的效果最佳。

Finding natural antioxidants for reparing skin photoaging is one of the research focuses at present.Poly-γ-glutamic acid(γ-PGA)is a natural polymer material prepared by microbial fermentation.γ-PGA has the ability of scavenging free radicals,but the relationship between the antioxidative effect,skin photoaging effect and molecular weight ofγ-PGA has not been clarified in this paper,the scavenging ability ofγ-PGA with different molecular weights on superoxide anion free radical,hydroxyl free radical and DPPH free radical was investigated.γ-PGA with different molecular weights exhibited scavenging activity on the above three free radicals with the scavenging rate of 31.36%,97.55%and 74.64%,respectively.In addition,by constructing ultraviolet-induced photoaging models in L929,γ-PGA with different molecular weights could repair photoaging cell damage and reduce ROS,NO in photoaging cells.γ-PGA with molecular weight 3.0×10^5γ-PGA had the best effect in repairing photoaging cell damage.