咪达唑仑对七氟烷麻醉诱发新生大鼠皮层异常脑电波的影响

Effect of midazolam on sevoflurane anesthesia-induced cortical abnormal electroencephalogram waves in neonatal rats

目的:评价咪达唑仑对七氟烷麻醉诱发新生大鼠皮层异常脑电波的影响。方法:清洁级健康雄性新生SD大鼠10只,7日龄,体重12~16 g。正确颅骨外植入电极板后,连续监测脑电图,吸入6%七氟烷3 min麻醉诱导。第1阶段:吸入2.1%七氟烷维持30 min;第2阶段:腹腔注射咪达唑仑3μg/g;第3阶段:吸入2.1%七氟烷维持60 min时腹腔注射氟马西尼10μg/g。每个阶段持续30 min。记录每个阶段惊厥波总时间和最后3 min棘波频率。结果:3个阶段惊厥波、棘波出现率和惊厥波总时间比较差异无统计学意义(P>0.05)。与第1和3阶段比较,第2阶段棘波频率降低(P<0.05)。结论:咪达唑仑可降低七氟烷麻醉诱发新生大鼠皮层棘波频率,但对惊厥波无抑制作用。

Objective To evaluate the effect of midazolam on sevoflurane anesthesia-induced cortical abnormal electroencephalogram waves in neonatal rats.Methods Ten male Sprague-Dawley rats,aged 7 days,weighing 12-16 g,were used in this study.After the electrodes were correctly implanted,the electroencephalogram was continuously monitored,and 6%sevoflurane was inhaled for 3 min to induce anesthesia.The experiment was carried out in three phases.In 1st phase,2.1%sevoflurane was inhaled for 30 min.In 2nd phase,midazolam 3μg/g was intraperitoneally injected.In 3rd phase,flumazenil 10μg/g was intraperitoneally injected at 60 min of 2.1%sevoflurane inhalation.Each phase lasted 30 min.The total time of convulsion wave in each phase and frequency of spike wave in the last 3 min of each phase were recorded.Results There was no significant difference in the rate of convulsion wave and spike wave developed and total duration of convulsion wave among the three phases(P>0.05).The frequency of spike wave was significantly decreased in 2nd phase compared with that in 1st and 3rd phases(P<0.05).Conclusion Midazolam can decrease the frequency of cortical spike waves induced by sevoflurane anesthesia,but produces no inhibitory effect on convulsion waves in the neonatal rats.