二次重度离心制备法对冷沉淀凝血因子质量的影响

Effect on the Quality of Cryoprecipitation from Fresh Frozen Plasma by High Speed Centrifugation Twice

目的探讨一次或二次重度离心制备新鲜冰冻血浆(冷沉淀凝血因子原料浆)对冷沉淀凝血因子质量的影响。方法检测冷沉淀凝血因子中游离血红蛋白含量、Ⅷ因子和纤维蛋白原含量。全血40单位(每单位200 mL)分成对照组、实验组,每组n=20。按国家标准采集、离心和分离,再将实验组新鲜冰冻血浆(冷沉淀凝血因子原料浆)进行第二次离心和分离,同时放入-80℃深低温冰箱速冻,储存时间为14天。14天后经过离心法制备成冷沉淀凝血因子,分别进行残留血红蛋白、Ⅷ因子和纤维蛋白原含量的检测。结果实验组残留血红蛋白含量(17.64±5.60)mg/L,显著低于对照组(25.75±9.78)mg/L(t=0.035,P<0.05);实验组Ⅷ因子含量(53.26±6.08)IU/单位,合格率100%高于对照组(47.34±9.14)IU/单位、合格率80%(t=0.041,P<0.05);实验组纤维蛋白原含量(162±29.01)mg/单位,高于对照组(147.28±43.39)mg/单位,两组合格率均100%(t=0.38,P>0.05)。结论二次重度离心新鲜冰冻血浆(冷沉淀凝血因子原料浆)能使残留红细胞更少,使残留游离血红蛋白更低,可以提高冷沉淀凝血因子中Ⅷ因子的含量,从而提高冷沉淀凝血因子制备质量。

Objective To study the effect on the quality of cryoprecipitation from fresh frozen plasma which was centrifugated with high speed once or twice.We detected the content of free hemoglobin,factorⅧand fibrinogen in cryoprecipitate.Methods 40 units whole blood(200 mL per-unit)were divided into two groups.The fresh frozen plasma in control group was centrifugated for once,and that in experimental group was centrifugated twice.Then the following separations were in accordance with national standards.We got the cryoprecipitate of two groups 14 days later,then detected the content of free hemoglobin,factorⅧand fibrinogen in cryoprecipitate.Results Free hemoglobin in the experimental group(17.64±5.60)mg/L is lower than that in the control group(25.75±9.78)mg/L,(P<0.05);and in the experimental group,the content of factorⅧis(53.26±6.08)IU/unit,which is higher than that in the control group(47.34±9.14)IU/unit(P<0.05);and the content of fibrinogen is(162±29.01)mg/unit,which is higher than that in the control group(147.28±43.39)mg/unit,(P>0.05).Conclusion By additional high speed centrifugation,more residual red blood cells can be removed from fresh frozen plasma,which may improve the quality of the cryoprecipitation,and increase the content of factorⅧand fibrinogen.