菟丝子黄酮对少弱精子症大鼠睾丸GM-CSF表达的影响

Cuscuta chinensis flavonoids reduce the expression of GM-CSF in the testis of oligozoospermia rats

目的:探究菟丝子黄酮对少弱精子症大鼠睾丸GM-CSF表达的影响。方法:SD雄性大鼠30只,随机分为空白对照组、模型组和菟丝子黄酮组,每组各10只,菟丝子黄酮组和模型组腹腔注射环磷酰胺,建立少弱精子症模型。菟丝子黄酮组灌服菟丝子黄酮混悬液,空白对照组和模型组灌服等量生理盐水,连续4周。取附睾液进行精子计数及活动率测定,制作睾丸切片并分别在光镜和电镜下观察睾丸组织学变化及超微结构改变。用蛋白芯片检测各组大鼠睾丸GM-CSF的表达情况。结果:模型组大鼠与空白对照组、菟丝子黄酮组大鼠相比,附睾液精子数目降低(P<0.01)且活动率下降(P<0.01),睾丸形态学发生明显改变,生精小管变形,生精细胞数目减少且排列混乱。菟丝子黄酮组大鼠的睾丸组织结构正常,精子数目、活动率与空白对照组的差异无统计学意义。菟丝子黄酮组和空白对照组大鼠睾丸GM-CSF的表达量均低于蛋白芯片可测得的最小值故而未测得准确数值,模型组大鼠睾丸GM-CSF表达显著上升。结论:环磷酰胺所致少弱精子症大鼠睾丸GM-CSF表达增强,经过菟丝子黄酮干预后GM-CSF表达与空白对照组的差异无统计学意义。

Objective:To investigate the effect of Cuscuta chinensis flavonoids(CCF)on the expression of the granulocyte-macrophage colony-stimulating factor(GM-CSF)in the testis of the rat with oligozoospermia(OZ).Methods:Thirty SD male rats were randomly divided into three groups of equal number,blank control,OZ model control and CCF intervention.The OZ model was established in the latter two groups by intraperitoneal injection of cyclophosphamide at 30 mg/kg qd for 5 successive days.From the 6 th day,the rats in the CCF intervention group were treated intragastrically with mixed suspension of CCF at 5 mL/kg and those in the other two groups with normal saline,all for 4 weeks.The epididymal sperm concentration and motility and the testicular morphology were examined and the expression of GM-CSF in the testis tissue detected with the SELDI Protein Chip.Results:Compared with the rats in the blank control and CCF intervention groups,the OZ model controls showed dramatically decreased epididymal sperm concentration and motility(both P<0.01)and significant morphological changes in the testis with deformed seminiferous tubules and reduced number and disordered arrangement of spermatogenic cells.Normal testicular morphology was observed in the CCF intervention group and there were no statistically significant differences in sperm concentration and motility between the CCF intervention and blank control groups(P>0.05).The expression of GM-CSF was significantly up-regulated in the testis tissue of the OZ model controls but lower than the minimum value obtained with the SELDI Protein Chip in the blank control and CCF intervention groups.Conclusion:Cuscuta chinensis flavonoids can significantly down-regulate the expression of GM-CSF in the testis of the rats with cyclophosphamide-induced oligozoospermia.