充氮包装和储藏温度对益生菌粉储藏稳定性的影响

Effects of nitrogen-filled packaging and storage temperature on storage stability of probiotics powder

考察了充氮包装和储藏温度对益生菌粉储藏稳定性的影响。将充氮和普通包装的动物双歧杆菌A6和长双歧杆菌BBMN68益生菌储藏于-20、4、25和37℃条件下12个月,检测其水分含量、水分活度及活菌数变化。结果表明,温度对益生菌粉稳定性影响较大,在冷冻和冷藏条件下,2种益生菌粉活菌数维持稳定,存活率均在85%以上,当储藏温度上升至常温及更高时,水分活度随着时间延长缓慢上升,而活菌数则下降较快,普通包装的A6和BBMN68益生菌粉在37℃储藏12个月益生菌存活率分别仅剩0.033%和0.013%,充氮包装的存活率分别为0.14%和0.094%,分别提升了4.2和7.2倍。所以益生菌粉最佳储藏条件是冷冻或冷藏,而充氮包装能够降低高温对稳定性的影响,特别是针对耐氧性较差的长双歧杆菌,作用效果更为显著。

This study evaluated the effects of nitrogen-filled packaging and storage temperature on the long-term storage stability of Bifidobacterium probiotics powder.The probiotics of Bifidobacterium animalis A6 and Bifidobacterium longum BBMN68 were stored at-20,4,25 and 37℃for 12 months,and the changes of moisture content,water activity and the number of viable cells were detected.The results showed that the storage temperature significantly affected the stability of probiotics powder.Under freezing and refrigeration conditions,the number of viable cells of the two probiotics powder remained stable,and the survival rates were all above 85%.When the storage temperature rose to normal temperature and higher,the water activity increased slowly,while the number of viable cells decreased rapidly.The survival rates of B.animalis A6 and B.longum BBMN68 probiotics powder stored in ordinary packaging at 37℃for 12 months were only 0.033%and 0.013%,but the survival rates of nitrogen-filled packaging were 0.14%and 0.094%,which increased by 4.2 times and 7.2 times respectively.Therefore,the optimal storage conditions of probiotics powder are freezing and refrigeration,and nitrogen-filled packaging can reduce the damage caused by high temperatures,especially for B.longum with poor oxygen resistance.