姜黄素联合多西他赛对宫颈癌hela细胞的增殖抑制及凋亡作用的研究

The Study of Antiproliferation and Apoptosis Effect of Curcumin Combined withDocetaxel on Human Cervical Hela Cells

目的研究姜黄素(Curcumin)增强多西他赛(Docetaxel)对人宫颈癌细胞(hela)抑制及凋亡作用。方法在体外姜黄素和多西他赛单独及联合使用作用于人宫颈癌细胞hela,MTT法检测增殖抑制情况,金氏Q值评价联合用药效应。流式细胞仪检测细胞凋亡情况。结果姜黄素及多西他赛均对人宫颈癌细胞(hela)有抑制作用;联合用药细胞抑制率明显增高,Q值分别为1.22(20μmol/ml姜黄素+1μg/ml多西他赛)和0.93(40μmol/ml姜黄素浓度+5μg/ml浅蓝菌素)。联合用药凋亡率较单药组明显增高(P<0.01)。结论姜黄素能增强多西他赛对人宫颈癌细胞(hela)的抑制及凋亡效应。

Objective To investigate antiproliferation and apoptosis effect of curcumin combined with docetaxel on human cervical hela cells.Methods Curcumin and docetaxel,alone or in combinataion,were given vitro to hela.The cells proliferation was measured by MTT analysis.The interaction between curcumin and docetaxel was assessed using the Jin's Q.Results Weather curcumin or docetaxel was able to inhibit the proliferation of cervical hela cells in vitro based on dose dependence.In combination group,the inhibition rate increased obviously.Q value were 1.24(when 20μmol/ml,Curcumin combined 1μg/ml Docetaxel)and 1.96(when 40μmol/ml,Curcumin combined 5μg/ml Docetaxel).The rate of apoptosis in the combined group was significantly higher than that in the single group(P<0.01).Conclusion Curcumin could enhance the inhibition of docetaxel on humanhuman cervical hela cells.