大肠癌BRAF基因突变与病理特征的相关性

Correlation between BRAF Gene Mutation and Pathological Features in ColorectalCancer

目的探讨大肠癌鼠类肉瘤滤过性毒菌致癌同源体B(BRAF)基因突变与病理特征的相关性。方法选择大肠癌患者118例与大肠腺瘤患者118例,取所有患者的病灶组织样本,采用实时荧光定量PCR法检测BRAF基因突变情况,调查患者的病例特征并进行相关性分析。结果大肠癌患者的BRAF基因突变率为34.7%,显著高于大肠腺瘤患者的5.1%(P<0.05)。在大肠癌患者中,不同性别、年龄、肿瘤直径、肿瘤部位、分化程度等患者的BRAF基因突变率对比差异无统计学意义(P>0.05),而不同浸润深度、淋巴结转移、临床分期等患者的BRAF基因突变率对比差异有统计学意义(P<0.05)。Cox比例风险模型显示:浸润深度、淋巴结转移、临床分期为导致BRAF基因突变的影响因素(P<0.05)。结论大肠癌BRAF基因突变率比较高,与患者的浸润深度、淋巴结转移、临床分期等病理特征显著相关。

Objective To investigate the relationship between the mutations of BRAF gene and pathological features in colorectal cancer.Methods 118 patients with colorectal cancer and 118 patients with colorectal adenoma were selected.The lesions of all patients were collected and the BRAF gene mutation were detected by real-time fluorescent quantitative PCR.The patient's case characteristics were investigated and given correlation analysis.Results The BRAF gene mutation rates in patients with colorectal cancer were 34.7%,which were significantly higher than that in patients with colorectal adenoma(P<0.05).In patients with colorectal cancer,there were no significant difference in BRAF gene mutation rates among patients with different gender,age,tumor diameter,tumor location,and differentiation degree(P>0.05),but patients with different invasive depth,lymph node metastasis,clinical stage,etc.were statistically significant difference in the BRAF gene mutation rates(P<0.05).The Cox proportional hazards model showed that the depth of invasion,lymph node metastasis,and clinical stage were the influencing factors of BRAF gene mutation(P<0.05).Conclusion The mutation rate of BRAF gene in colorectal cancer is relatively high,which is significantly correlated with pathological features such as depth of invasion,lymph node metastasis and clinical stage.