用于循环肿瘤细胞分选富集的双螺旋微流控芯片研究

Study on double spiral microfluidic chip for sorting and enriching circulating tumor cells

本研究开发了一种双螺旋结构的微流控芯片,采用Ansys fluent软件进行仿真模拟,用Solidworks软件绘图设计,用SU-8光刻胶模具制作芯片,并使用含有循环肿瘤细胞的红细胞裂解后的血液样品,探究芯片的分选效率。试验结果表明,样品的不同流速影响芯片对循环肿瘤细胞的分选效果,在30 mL/h的流速下,微流控芯片的分选率最高,达到77%。本研究结果对临床肿瘤的诊断具有重要意义。

We developed a microfluidic chip with double spiral structure.The software Ansys fluent was used for simulation,SolidWorks was used for graphic design,the chip was made with SU-8 photoresist mold,and the blood samples of red blood cells containing circulating tumor cells were used to explore the sorting efficiency of the chip.The results showed that different flow rates of samples affected the sorting effect of microfluidic chip on circulating tumor cells.At the flow rate of 30mL/h,the sorting rate of microfluidic chip was the highest,reached 77%.The results are of great significance for the diagnosis of clinical tumors.