S100A4蛋白表达与结直肠癌的关系研究

Study on the relationship between S100A4 protein expression and colorectal cancer

目的探讨转移相关蛋白S100A4表达与结直肠癌的关系。方法选取2017年3月—2019年3月石家庄市第三医院和衡水市第四人民医院手术切除的结直肠癌肿瘤标本74份作为研究组,同时选取经病理证实正常结直肠组织40份作为对照组。比较2组中肿瘤转移抑制基因nm23H1、S100A4蛋白表达情况,分析结直肠癌组织中nm23H1、S100A4阳性表达情况与临床病理特征间的关系以及S100A4与nm23H1蛋白表达的关系。结果S100A4主要存在于细胞核中且表达为棕褐色或棕黄色,研究组中S100A4阳性率明显高于对照组(P<0.05)。nm23H1主要存在于细胞质中且为棕黄色细颗粒,研究组中nm23H1阳性率明显低于对照组(P<0.05);在结直肠癌组织中,nm23H1阳性表达率与患者肿瘤大小、肝转移、年龄、组织分化程度、发生部位、性别无明显相关性(P均>0.05),与肿瘤浸润深度、有无淋巴结转移、TNM分期均有显著相关性(P均<0.05),且与TNM分期呈正相关。在结直肠癌组织中,S100A4阳性表达率与患者肿瘤大小、肝转移、年龄、TNM分期、发生部位、性别无明显相关性(P均>0.05),与肿瘤浸润深度、有无淋巴结转移、组织分化程度有明显相关性(P均<0.05),且与组织分化程度呈负相关;结直肠癌组织中S100A4表达与nm23H1表达存在负相关性(r=-0.432,P<0.05)。结论S100A4蛋白表达情况与淋巴结转移、病理分期密切相关,其与nm23H1基因联合检测,有利于明确患者转移、浸润、预后情况。

Objective It is to investigate the relationship between the expression of metastasis-associated protein(S100A4)and colorectal cancer.Methods A total of 74 colorectal cancer tumor specimens from the Third Hospital of Shijiazhuang and the Fourth People’s Hospital of Hengshui from March 2017 to March 2019 were selected as the study group,and 40 normal colorectal tissues confirmed by pathology were selected as the control group.The expression of tumor metastasis suppressor gene(nm23H1)and S100A4 protein in the two groups were compared,and the relationship between the positive expression of nm23H1 and S100A4 in colorectal cancer tissues and clinicopathological characteristics and the relationship between S100A4 and nm23H1 protein expression were analyzed.Results S100A4 mainly existed in the cell nucleus and the expression was brown yellow or brown.The positive rate of S100A4 in the study group was significantly higher than that in the control group(P<0.05).Nm23H1 mainly existed in the cytoplasm and was brown-yellow fine particles.The positive rate of nm23H1 in the study group was significantly lower than that in the control group(P<0.05).In colorectal cancer,the positive expression rate of nm23H1 was not related to the size of tumor,liver metastasis,age,tissue differentiation,the location of occurrence,and gender(P>0.05),but significantly correlated with the depth of tumor invasion,the presence or absence of lymph node metastasis,and TNM classification(P<0.05),and it was positively correlated with TNM classification.In colorectal cancer tissues,the positive expression rate of S100A4 was not significantly correlated with tumor size,liver metastasis,age,TNM classification,location of occurrence,and gender(P>0.05),but was significantly correlated with the depth of tumor invasion,lymph node metastasis,and tissue differentiation(P<0.05),and was negatively correlated with the degree of tissue differentiation;S100A4 expression in colorectal cancer tissue was negatively correlated with nm23H1 expression(r=-0.432,P<0.05).Conclusion The expression of S100A4 protein is closely related to lymph node metastasis and pathological classification.The combined detection of S100A4 protein and nm23H1 gene is helpful to clarify the metastasis,invasion and prognosis of patients with colorectal cancer.