HPLC同时测定感冒解毒灵颗粒中8种有效成分的含量

Simultaneous Determination of Eight Components in Ganmao Jieduling Granules by HPLC

目的建立高效液相色谱法同时测定感冒解毒灵颗粒中(R,S)-告依春、新绿原酸、绿原酸、升麻素苷、5-O-甲基维斯阿米醇苷、橙皮苷、连翘苷和牛蒡苷8种有效成分的含量。方法采用SynergiTM Hydro-RP 80Ȧ色谱柱(250 mm×4.6 mm,4μm),流动相为乙腈-0.1%磷酸(梯度洗脱),流速为1.0 ml/min,柱温35℃。结果8种成分分离良好;各成分的进样质量范围分别在0.5485~21.94 ng(r=0.9998)、8.070~322.8 ng(r=0.9998)、19.67~786.8 ng(r=0.9999)、20.89~835.8 ng(r=0.9999)、6.400~256.0 ng(r=0.9996)、163.3~6531 ng(r=0.9998)、48.02~1921 ng(r=0.9999)、33.00~1320 ng(r=0.9998)范围内与峰面积呈良好的线性关系;加样回收率分别为99.76%,99.43%,99.90%,97.84%,99.62%,99.25%,98.67%,98.10%,RSD分别为1.80%,1.04%,1.59%,1.77%,1.73%,0.58%,1.49%,1.50%。结论此法简单、快速、灵敏、准确,可用于同时测定感冒解毒灵颗粒8个指标性成分。

Objective To develop an HPLC method for the simultaneous determination of(R,S)-goitrin,neochlorogenic acid,chlorogenic acid,prim-O-glucosylcimifugin,5-O-methylvisammioside,hesperidin,forsythin and arctiin in Ganmao Jieduling Granules.Methods The chromatographic separation was performed on a SynergiTM Hydro-RP 80Ȧcolumn(250 mm×4.6 mm,4μm)with gradient elution by acetonitrile-0.1%phosphoric acid at the flow rate of 1.0 ml/min.The column temperature was 35℃.Results The ranges for linear correlation of(R,S)-goitrin,neochlorogenic acid,chlorogenic acid,prim-O-glucosylcimifugin,5-O-methylvisammioside,hesperidin,forsythin and arctiin were 0.5485-21.94 ng(r=0.9998),8.070-322.8 ng(r=0.9998),19.67-786.8 ng(r=0.9999),20.89-835.8 ng(r=0.9999),6.400-256.0 ng(r=0.9996),163.3-6531 ng(r=0.9998),48.02-1921 ng(r=0.9999)and 33.00-1320 ng(r=0.9998),respectively.The average recoveries were 99.76%,99.43%,99.90%,97.84%,99.62%,99.25%,98.67%and 98.10%,with RSDs of 1.80%,1.04%,1.59%,1.77%,1.73%,0.58%,1.49%and 1.50%,respectively.Conclusion This method is simple,rapid,sensitive and accurate,and can be used to determine the eight components in Ganmao Jieduling Granules simultaneously.