沼泽红假单胞菌在蓝藻基质中的生长潜势和碳氮磷利用

Growth potential and CNP sequestration of Rhodopseudomonas palustris in cyanobacterial substrate.

为同步高效利用废弃蓝藻和产出活性光合细菌,研究了蓝藻基质中沼泽红假单胞菌(Rhodopseudomonas palustris)的生长潜势和碳氮磷转化规律.结果表明,温度30℃,光照2000lx,蓝藻浓度>0.87g/L,即可促进R.palustris PUF1生长;蓝藻浓度3g/L,培养108h,PUF1OD650虽低于ATYP培养基,但细菌叶绿素a(Bchl a)浓度与ATYP基质相当,表明蓝藻基质可能促进了PUF1光合色素的合成.在细菌对数期后期补加小分子有机酸显著促进蓝藻基质中PUF1再生长,252,444h OD650分别是培养108h的191.75%和269.24%,Bchl a分别是108h的206.68%和276.17%.444h蓝藻基质中OD650和Bchl a分别是ATYP基质中的130.88%和160.62%,表明长期培养条件下应用天然蓝藻基质更适宜活性PUF1培育.通过分析干物质和藻液中的碳氮磷含量和浓度发现,蓝藻基质中可供给PUF1直接利用的溶解性碳氮磷浓度不平衡,表现为氮充足,碳少量,磷限制.提高蓝藻基质中生物可利用碳浓度对强化光合细菌生长和碳氮磷利用具有重要意义.

In order to efficiently use wasted cyanobacteria and simultaneously yield vigorous phototrophic bacteria,the growth potential and transformation of carbon,nitrogen and phosphorus of Rhodopseudomonas palustris in cyanobacterial substrates were determined.A cyanobacterial concentration greater than 0.87g/L can stimulate the proliferation of R.palustris PUF1under the condition of 30℃,2000lx.Though less of OD650 in cyanobacterial substrates (3g/L) than that of the ATYP medium at 108h,the concentration of bacteriochlorophyll a (Bchl a) was in similarity showing the more active synthesis of photopigments in cyanobacterial substrates.The addition of smaller molecular organic acids significantly regrew up the PUF1cells in late log phase.Specifically,at 252h and 444h,OD650 in cyanobacterial substrates was enhanced by 191.75% and 269.24%,while Bchl a was 206.68% and 276.17%,respectively.At 444h,OD650 and Bchl a in cyanobacterial substrates were separately 130.88% and 160.62% of the ATYP medium,indicating the superiority of natural cyanobacterial substrate to the ATYP medium for grown PUF1s,especially under a long incubation period.Evaluating the dried biomaterials and dissolved nutrients in cyanobacterial substrates,it indicated the unbalanced distribution of bioavailable carbon,nitrogen and phosphorus,namely surplus nitrogen,lacked carbon and limited phosphorus.It matters of improved bioavailable carbon in cyanobacterial biomasses for the enhanced growth of PUF1and nutrient sequestration.