甲型H1N1流感73例T淋巴细胞亚群分析

T cell immunity analysis in patients with influenza A virus subtype H1N1

目的检测甲型H1N1流感病人外周血T淋巴细胞亚群的变化,探讨甲型H1N1流感对其细胞免疫功能的影响。方法总结2009年11月5日至2017年1月15日甘肃省人民医院及兰州大学第二医院所收治的73例临床资料完整且没有基础免疫疾病的甲型H1N1流感病人血清总T淋巴细胞、CD4抗原阳性的T淋巴细胞(CD4+T淋巴细胞)、CD8抗原阳性的T淋巴细胞(CD8+T淋巴细胞)活性的变化规律。结果甲型H1N1流感病人CD8+T淋巴细胞在诊断初期即已升高(42.27±11.13)cells/ul,并在恢复期基本恢复正常(29.26±12.99)cells/ul;总T淋巴细胞、CD4+T淋巴细胞则在诊断初期有不同程度下降(44.09±13.51)、(47.27±7.73)cells/ul,并随病情好转逐渐恢复,CD4+T淋巴细胞与CD8+T淋巴细胞比值在病程中没有统计学差异差异无统计学意义(F=0.294,0.415,0.618;P=0.892,0.106,0.082),但随病情好转有升高趋势。结论甲型H1N1流感病人免疫功能低下。

Objective changes of CD4 antigen positive T lymphocytes(CD4+T cells),CD8 antigen positive T lymphocytes(CD8+T cells)and CD4+/CD8+in 73 patients with A H1 N1 influenza with complete clinical data and no underlying immune diseases were analyzed.From November 5,2009 to January 15,2017,the patients in Gansu Provincial People’s Hospital and the Second Hospital of Lanzhou University were treated.ResultsCD8+T lymphocytes in patients with influenza A(H1 N1)increased at the early stage of diagnosis(42.27±11.13 cells/uL),and returned to normal in the recovery period(29.26±12.99 cells/uL);the total T lymphocytes and CD4+T lymphocytes decreased to varying degrees(44.09±13.51 cells/uL,47.27±7.73 cells/uL,respectively),and gradually recovered with the improvement of the disease.There was no significant difference between the two groups(F=0.294,0.415,0.618;P=0.892,0.106,0.082),but it increased with the improvement of the disease.ConclusionT cell immunity are impaired in patients with a H1 N1 influenza.