肿瘤浸润性树突状细胞在口腔部鳞状细胞癌组织中的表达及价值

Expression and value of tumor infiltrating dendritic cells in oral squamous cell carcinoma

目的探讨口腔部鳞状细胞癌病人癌组织中肿瘤浸润性树突状细胞的表达及其价值。方法选择2017年6月至2018年6月河北省儿童医院收治的50例口腔部鳞状细胞癌病人作为观察组,选取50例同时期来该院进行治疗的口腔良性肿物病人作为本次研究的对照组。取两组病人的肿物组织标本进行免疫组织化学染色法分析肿瘤浸润性树突状细胞密度,采用流式细胞仪计数肿瘤浸润性树突状细胞的人主要组织相容性复合体Ⅱ类(MHC-Ⅱ)阳性细胞比例和白细胞抗原54(CD54)阳性细胞比例。结果观察组肿瘤肿瘤淋巴结转移分类(TNM)分期为Ⅲ期的肿瘤浸润性树突状细胞密度、MHC-Ⅱ阳性肿瘤浸润性树突状细胞比例及CD54阳性肿瘤浸润性树突状细胞比例均明显低于Ⅰ/Ⅱ期[(7.61±1.43)比(8.65±1.42),P=0.014;(5.31±1.80)%比(7.46±1.95)%,P=0.000;(6.62±2.11)比(8.21±1.86)%,P=0.008];肿瘤组织的最大直径≥5 cm的肿瘤浸润性树突状细胞密度、MHC-Ⅱ阳性肿瘤浸润性树突状细胞比例及CD54阳性肿瘤浸润性树突状细胞比例均明显低于肿瘤组织直径<5 cm的病人[(7.14±1.85)比(9.07±2.11),P=0.001;(5.42±1.84)比(7.73±1.85),P=0.000;(6.34±2.06)比(8.47±1.91),P=0.000];观察组肿瘤分化程度不同的病人上述指标相比均差异无统计学意义(P>0.05);观察组肿瘤浸润性树突状细胞密度明显低于对照组[(8.27±2.11)比(9.68±2.38),P=0.002],MHC-Ⅱ阳性肿瘤浸润性树突状细胞比例明显低于对照组[(6.67±2.20)比(9.79±2.58),P=0.000],CD54阳性肿瘤浸润性树突状细胞比例明显低于对照组[(7.46±2.31)比(9.83±2.54),P=0.000]。结论口腔部鳞状细胞癌病人癌组织内肿瘤浸润性树突状细胞的密度下降、MHC-Ⅱ阳性及CD54阳性的细胞比例降低,且随着TNM分期及肿瘤直径的增大,肿瘤浸润性树突状细胞的表达水平更低。

Objective To investigate the expression and value of invasive dendritic cells in oral squamous cell carcinoma(SCC).MethodFifty patients with oral squamous cell carcinoma admitted to Hebei Provincial Children’s Hospital from June 2017 to June2018 were selected as the observation group,and 50 patients with benign oral masses treated in our hospital during the same period were selected as the control group.The neoplasm tissue samples of patients in the two groups were taken for immunohistochemical staining to analyze the density of tumor infiltrating dendritic cell,and flow cytometry was used for counting of tumor infiltrating dendritic cells MHC-Ⅱpositive cells and CD54 positive cells.ResultIn the observation group,the density of infiltrating dendritic cells,the proportion of MHC-Ⅱ-positive tumor infiltrating dendritic cells,and the proportion of CD54-positive tumor infiltrating dendritic cells in tumors with tumor lymph node metastasis classification(TNM)stage III were significantly lower than those withⅠ/Ⅱstage[(7.61±1.43)vs.(8.65±1.42),P=0.014,(5.31±1.80)%vs.(7.46±1.95)%,P=0.000,and(6.62±2.11)%vs.(8.21±1.86)%,P=0.008];the tumor infiltrating dendritic cell density,MHC-Ⅱpositive cell rate and CD54 positive cells in tumor with the maximum diameter of≥5 cm were significantly lower than those of patients with tumor diameter<5 cm[(7.14±1.85)vs.(9.07±2.11),P=0.001,(5.42±1.84)vs.(7.73±1.85),P=0.000 and(6.34±2.06)vs.(8.47±1.91),P=0.000];There was no significant difference in the above indicators among patients with different degrees of tumor differentiation in the observation group(P>0.05).Tumor infiltrating dendritic cells density of observation group was obviously lower than that of the control group(8.27±2.11)vs.(9.68±2.38),P=0.002,percentage of MHC-Ⅱpositive cell significantly lower than the control group(6.67±2.20)vs.(9.79±2.58),P=0.000,percentage of CD54 positive cell significantly lower than the control group(7.46±2.31)vs.(9.83±2.54),P=0.000.Conclusion The density of tumor-infiltrating dendritic cells in the cancer tissue of patients with oral squamous cell carcinoma decreased,and the proportion of MHCⅡ-positive and CD54-positive cells decreased.With the increase of TNM staging and tumor diameter,the expression level of tumor infiltrating dendritic cells is lower.