维吾尔药复方待比地力对顺铂所致小鼠肾损伤的保护作用

Protective effects of DaiBiDiLi,a traditional Uighur prescription,on cisplatin-induced renal injury in mice

目的:观察维吾尔药复方待比地力(DBD)对顺铂所致小鼠肾损伤的影响.方法:昆明小鼠40只随机分为正常组、肾损伤模型组、DBD低和高剂量干预组共4组.检测小鼠血清尿素氮(BUN)和肌酐(Cr)含量以及肾脏MDA、GSH、SOD和CAT、TNF-α、IL-1β和IL-6水平;观察肾脏显微结构;并检测肾内NOD样受体蛋白3(NLRP3)、凋亡相关斑点样蛋白(ASC)、半胱氨酸天冬氨酸蛋白酶-1(caspase-1)和IL-1β的蛋白表达水平.结果:DBD能显著下调肾损伤小鼠血清中BUN和Cr的含量并改善顺铂诱导的肾脏显微结构变化.与肾损伤模型组比较,DBD组小鼠肾内MDA水平明显降低,GSH、SOD和CAT水平则明显增加.顺铂诱导了NLRP3、ASC和caspase-1蛋白的表达并上调肾内炎症因子TNF-α、IL-1β和IL-6的含量.DBD剂量依赖性地改善了小鼠肾损伤.结论:DBD能明显缓解顺铂诱导的小鼠肾损伤,其机制可能与通过抑制肾内NLRP3炎症小体各组分的表达来减轻顺铂诱导的肾脏炎症反应有关.

Objective:To observe the effect of a traditional Uighur prescription,DaiBiDiLi(DBD),on acute kidney injury in mice induced by cisplatin(CP).Methods:Forty Kunming mice were randomly divided into 4 groups,i.e.,the normal group,the model group,the DBD low-dosage group and the DBD high-dosage group.Serum and kidneys were collected immediately.Levels of urea nitrogen(BUN),creatinine(Cr),MDA,rGSH,SOD,catalase(CAT)TNF-α,IL-1β,and interleukin-6(IL-6)were detected.HE staining was performed to observe the microstructure of kidneys.Protein expression levels of nod-like receptor protein 3(NLRP3),apoptosis-related spot-like protein(ASC),caspase-1,and IL-1βwere determined also.Results:DBD significantly reduced levels of BUN and Cr in serum and improved the changes of renal microstructure induced by CP.The level of MDA in the kidney of DBD groups declined significantly,while levels of GSH,SOD,and CAT increased considerably.CP induced the expression of NLRP3,ASC,and caspase-1 in kidney and upregulated renal contents of TNF-α,IL-1βand IL-6.DBD ameliorated these lesions in a dose-dependent manner.Conclusion:DBD significantly alleviated CP-induced kidney injury in mice.Inhibition of the expression of NLRP3 inflammasome components to decline CP-provoked renal inflammation might be implicated in the kidney protective effect of DBD.