HBx-MALAT-XB130通路对肝癌细胞生长和迁移的影响机制

The mechanism of the influence of HBx-MALAT-XB130 pathway on the growth and migration of liver cancer cells

目的探讨HBx-MALAT-XB130通路对肝癌细胞生长和迁移的影响机制。方法2019年6—10月于解放军联勤保障部队第962医院实验室进行实验,选取人肝癌细胞株,培养后分为低阻断组、中阻断组、高阻断组,分别加入r-分泌酶抑制剂(DAPT)1 ml、5 ml、10 ml处理。MTT法检测各组细胞生长能力,TUNEL法检测细胞凋亡情况,流式细胞仪检测细胞周期分布情况,使用Transwell小室实验检测细胞侵袭情况。结果培养24 h、48 h、72 h时人肝癌细胞生长率比较,低阻断组<中阻断组<高阻断组(F=15.683、24.658、28.667,P均<0.01),细胞凋亡率比较,低阻断组>中阻断组>高阻断组(F=16.856、22.337、18.697,P均<0.01);细胞侵袭和迁移能力比较,低阻断组<中阻断组<高阻断组(F=35.664、28.553,P均<0.01);于G1、S、G2期肝癌细胞比例比较,高阻断组>中阻断组>低阻断组(F=9.583、13.521、16.552,P均<0.01);Bcl-2相对表达量低阻断组>中阻断组>高阻断组,而Bax、Caspase3、p53相对表达量低阻断组<中阻断组<高阻断组(F=11.234、15.367、12.367、10.952,P均<0.01)。结论HBx-MALAT-XB130通路与肝癌细胞生长率、凋亡、侵袭、迁移和肝癌细胞的细胞同期分布情况有着密切的联系,能够为肝癌的临床治疗起到一定的参考作用。

Objective To explore the mechanism of HBx-MALAT-XB130 pathway on the growth and migration of liver cancer cells.Methods From June to October 2019,experiments were conducted in the laboratory of the 962th Hospital of the Joint Logistics Support Force of the People's Liberation Army.Human liver cancer cell lines were selected and divided into low-blocking group,medium-blocking group,and high-blocking group after culture,and rsecretase was added respectively Inhibitor(DAPT)1 ml,5 ml,10 ml treatment.MTT method was used to detect cell growth ability in each group,TUNEL method was used to detect cell apoptosis,flow cytometry was used to detect cell cycle distribution,and the Transwell chamber experiment was used to detect cell invasion.Results Comparison of the growth rate of human hepatocarcinoma cells when cultured for 24 h,48 h,and 72 h,the low blocking group<the medium blocking group<the high blocking group(F=15.683,24.658,28.667,P<0.01),the apoptosis rate Comparison,low blocking group>medium blocking group>high blocking group(F=16.856,22.337,18.697,P<0.01);comparison of cell invasion and migration ability,low blocking group<medium blocking group<high blocking group Interrupted group(F=35.664,28.553,P<0.01);comparison of the proportions of liver cancer cells in G1,S,and G2 stages,high-block group>medium block group>low-block group(F=9.583,13.521,16.552,P<0.01);Bcl-2 relative expression level low block group>medium block group>high block group,while Bax,Caspase3,p53 relative expression level low block group<medium block group<high block group(F=11.234,15.367,12.367,10.952,all P<0.01).Conclusion The HBx-MALAT-XB130 pathway is closely related to the growth rate,apoptosis,invasion,migration of liver cancer cells and the distribution of liver cancer cells at the same time,which can play a certain reference role for the clinical treatment of liver cancer.