泛素偶联酶E2C在原发性肝癌中的表达及与临床病理参数和预后的关系

The expression of ubiquitin coupling enzyme E2C in primary liver cancer and its relationship with clinicopathological parameters and prognosis

目的分析泛素偶联酶E2C(UBE2C)在原发性肝癌中的表达及与临床病理参数和预后的关系。方法选取2014年1月—2016年12月北部战区总医院普外科手术治疗原发性肝癌患者109例作为研究对象,留取其癌组织和癌旁组织。采用免疫组化法测定原发性肝癌组织和癌旁组织中UBE2C表达水平,分析其与原发性肝癌临床病理参数及患者预后的关系。结果原发性肝癌组织中UBE2C阳性表达率高于癌旁组织(92.16%vs.64.22%,χ^2/P=26.067/0.000)。UBE2C在原发性肝癌组织中的表达水平与患者的性别、年龄、肿瘤大小、浸润深度、淋巴结转移、分化程度无相关性(P>0.05),与TNM分期、淋巴血管间隙浸润、复发呈正相关(r/P=0.506/0.000、0.517/0.000、0.493/0.002)。术后随访,UBE2C高表达原发性肝癌患者中位生存时间为23.32个月,短于UBE2C低表达患者的33.32个月(χ^2/P=9.129/0.003)。COX多因素分析表明,有淋巴血管间隙浸润、复发、UBE2C高表达是影响原发性肝癌患者不良预后的独立危险因素[OR(95%CI)=2.880(1.348~6.153)、2.975(1.091~8.115)、3.475(1.942~6.219)]。结论UBE2C在原发性肝癌组织中阳性表达率显著升高,与患者TNM分期、淋巴血管间隙浸润、复发相关,可能成为评价原发性肝癌患者预后的临床指标。

Objective To analyze the expression of ubiquitin coupling enzyme E2C(UBE2C)in primary liver cancer and its relationship with clinicopathological parameters and prognosis.Methods From January 2014 to December 2016,109 patients with primary liver cancer treated by general surgery in the General Hospital of the Northern Theater Command were selected as the research objects,and their cancerous and para-cancerous tissues were retained.Immunohistochemical method was used to determine the expression level of UBE2C in primary liver cancer tissues and adjacent tissues,and analyze its relationship with the clinicopathological parameters of primary liver cancer and patient prognosis.Results The positive expression rate of UBE2C in primary liver cancer tissues was higher than that in adjacent tissues(92.16%vs.64.22%,χ^2/P=26.067/0.000).The expression level of UBE2C in primary liver cancer tissues has no correlation with the patient’s gender,age,tumor size,depth of invasion,lymph node metastasis,and degree of differentiation(P>0.05),but is positively correlated with TNM staging,lymphatic vascular space invasion,and recurrence(r/P=0.506/0.000,0.517/0.000,0.493/0.002).After follow-up,the median survival time of patients with primary liver cancer with high UBE2C expression was 23.32 months,which was shorter than 33.32 months for patients with low UBE2C expression(χ^2/P=9.129/0.003).COX multivariate analysis showed that lymphovascular space infiltration,recurrence,and high UBE2C expression are independent risk factors that affect the poor prognosis of patients with primary liver cancer[OR(95%CI)=2.880(1.348-6.153),2.975(1.091~8.115),3.475(1.942~6.219)].Conclusion The positive expression rate of UBE2C in primary liver cancer tissues is significantly increased,which is related to the TNM staging,lymphatic and vascular space infiltration and recurrence of patients,and may become a clinical indicator for evaluating the prognosis of patients with primary liver cancer.