髓过氧化物酶/对氧磷酶1与高密度脂蛋白功能的关系

Study on the relationship between myeloperoxidase/paraoxonase 1 and high density lipoprotein function

目的探讨冠心病患者髓过氧化物酶(MPO)/对氧磷酶1(PON1)与高密度脂蛋白(HDL)功能的关系及槲皮素对HDL功能的保护作用。方法分别选取150名AMI和非冠心病住院患者,收集基本临床资料、生化指标及空腹静脉血,测定血浆中MPO和PON1的水平。培养人单核细胞(THP-1),并诱导为巨噬细胞,分别用来源于AMI和非冠心病患者apoB缺乏的血清干预实验,测定胆固醇流出率、ABCA1和ABCG1蛋白和mRNA的表达、炎症因子TNF-α、IL-6的水平。结果AMI组血浆中MPO水平明显高于非CHD组,PON1则明显低于非CHD组,两者比值MPO/PON1为AMI组小于非CHD组(P<0.001)。4组间胆固醇流出率、ABCA1和ABCG1的蛋白及mRNA的表达、炎症因子TNF-α、IL-6水平的差异均有统计学意义。结论AMI患者的HDL功能发生了改变,MPO/PON1水平可能间接反映HDL的胆固醇逆转运功能,槲皮素可以改善胆固醇的逆转运功能和巨噬细胞内的炎症反应。

Objective To investigate the relationship between MPO/PON1 and HDL in patients with coronary heart disease and the protective effect of quercetin on HDL function.Methods 150 patients with AMI and non CHD were selected.The basic clinical data,biochemical indexes and fasting venous blood were collected to determine the levels of MPO and PON1 in plasma.Human monocytes(THP 1)were cultured and induced into macrophages.They were used in serum intervention experiments of apoB deficiency in patients with AMI and non coronary heart disease.The cholesterol efflux rate,the expression of ABCA 1 and ABCG1 protein and mRNA,and the levels of inflammatory factors TNFαand IL 6 were measured.Results The level of MPO in AMI group was significantly higher than that in non CHD group,while PON 1 was significantly lower than that in non CHD group.The ratio of MPO/PON1 was lower in AMI group than that in non CHD group(P<0.001).There were significant differences in cholesterol efflux rate,protein and mRNA expression of ABCA1 and ABCG1,and levels of inflammatory factors TNFαand IL 6 among the four groups.Conclusion The results suggests the functions of HDL in patients with CHD is damaged.MPO/PON1 level may indirectly reflect the HDL reverse cholesterol transport function,quercetin can improved reverse cholesterol transport function and inflammatory responses in macrophages.