弥漫大B细胞淋巴瘤组织中LncRNA TP73-AS1的检测水平及临床意义

Detection level and clinical significance of LncRNA TP73-AS1 in diffuse large B-cell lymphoma

目的探讨弥漫大B细胞淋巴瘤(DLBCL)组织中长链非编码RNA(LncRNA)p53依赖性凋亡调节物(TP73-AS1)的检测水平与临床病理特征及预后的关系。方法选取2010年1月~2014年3月四川大学华西医院109例DLBCL患者为DLBCL组,同期110例淋巴增生患者作为对照组,实时荧光定量(qRT-PCR)法检测组织中LncRNA TP73-AS1表达水平。分析其与患者性别、年龄、发病部位、Ann Arbor分期、骨髓侵犯情况、美国东部肿瘤协作组(ECOG)体力状况评分、全身(B)症状、国际预后指数(IPI)评分、乳酸脱氢酶(LDH)水平、组织学形态的关系,Kaplan-Meier生存曲线分析LncRNA TP73-AS1对DLBCL患者生存情况的影响,COX分析影响DLBCL患者预后的因素。结果与对照组相比,DLBCL组组织中LncRNA TP73-AS1水平升高(P<0.05)。与LncRNA TP73-AS1低表达组相比,高表达组年龄(≥60)、部位(结内)、Ann Arbor分期(Ⅲ-Ⅳ)、ECOG评分(>1分)、B症状(有)、IPI评分(中高危)、LDH(异常)升高(P<0.05),生存率降低(P<0.05)。LncRNA TP73-AS1高表达、Ann Arbor分期(Ⅲ-Ⅳ)、ECOG评分(>1分)、B症状(有)是影响BLBCL患者预后不良的独立危险因素。结论DLBCL组织中LncRNA TP73-AS1水平升高,LncRNA TP73-AS1高表达与预后不良关系密切,LncRNA TP73-AS1可作为预测DLBCL预后不良的潜在标志物。

Objective To detect the level of long non-coding RNA(LncRNA)p53 dependent apoptosis regulator(TP73-AS1)in diffuse large B-cell lymphoma(DLBCL)and to explore its relationships with clinicopathological characteristics and prognosis.Methods From January 2010 to March 2014,109 patients with DLBCL in West China Hospital of Sichuan University were selected as the study objects(DLBCL group),and 110 patients with lymphoproliferative disease were selected as the controls(control group),the expression level of LncRNA TP73-AS1 was detected by real-time fluorescence quantitative analysis(qRT-PCR).Its relationships with patients’gender,age,location of disease,Ann Arbor stage,bone marrow invasion,ECoG physical condition score,systemic(B)symptoms,international prognostic index(IPI)score,lactate dehydrogenase(LDH)level and histological morphology,Kaplan Meier survival curve was used to analyze the effect of LncRNA TP73-AS1 on the survival of patients with DLBCL,and COX was used to analyzed the prognostic factors in patients with DLBCL.Results Compared with the control group,the level of LncRNA TP73-AS1 in DLBCL group increased(P<0.05).Compared with the low expression group of LncRNA TP73-AS1,the age(≥60),site(intranodal),Ann Arbor stage(Ⅲ-Ⅳ),ECOG score(>1),B symptom(yes),IPI score(medium and high risk),LDH(abnormal)in the high expression group increased(P<0.05),and the survival rate decreased(P<0.05).High expression of LncRNA TP73-AS1,Ann Arbor stage(Ⅲ-Ⅳ),ECOG score(>1 point),B symptom(yes)were independent risk factors for poor prognosis in patients with BLBCL.Conclusion The level of LncRNA TP73-AS1 in DLBCL is increased,high expression of LncRNA TP73-AS1 is closely related to poor prognosis,LncRNA TP73-AS1 can be used as a potential marker to predict the poor prognosis of DLBCL.