林下参内生球毛壳菌FS-01对人参病原真菌的抑制作用

Inhibitory effect of Chaetomium globosum FS-01 from mountain-cultivated ginseng against ginseng pathogens

为探究林下参内生真菌球毛壳菌(Chaetomium globosum)FS-01菌株对人参病原菌的抑菌作用,该研究在实验室条件下,测定了FS-01菌株菌丝、发酵液和孢子悬浮液对人参黑斑病菌(Alternaria panax)、人参菌核病菌(Sclerotinia schinseng)、人参灰霉病菌(Botrytis cinerea)、人参立枯病菌(Rhizoctonia solani)、人参根腐病菌(Fusarium solani)5种人参病原菌的抑制作用。结果表明:内生真菌球毛壳菌FS-01对5种病原菌均有抑制作用,其中,对人参黑斑病菌的抑制作用最高,为30.80%,其次是人参立枯病菌、人参菌核病菌、人参根腐病菌和人参灰霉病菌;发酵液抑菌实验结果表明,在加入内生真菌球毛壳菌FS-01菌株发酵液的PDA培养基上,对人参灰霉病菌的抑制作用最高,为82.09%,其次是人参菌核病菌、人参黑斑病菌、人参立枯病菌和人参根腐病菌;孢子抑菌实验结果表明,在加入内生真菌球毛壳菌FS-01菌株孢子悬浮液的PDA培养基上,对人参黑斑病菌的抑制作用最高,为83.72%,其次是人参灰霉病菌、人参立枯病菌、人参菌核病菌和人参根腐病菌。综上结果认为,内生真菌球毛壳菌FS-01菌株对人参病原菌均有很高的抑菌作用,可作为人参病原菌的生防菌株资源。

To study the inhibitory effects of Chaetomium globosum strain FS-01 against Alternaria panax,Sclerotinia schinseng,Botrytis cinerea,Rhizoctonia solani and Fusarium solani of ginseng pathogens,mycelia,fermentation broth and conidia suspension were tested in laboratory.The results showed that Chaetomium globosum FS-01 had certain inhibitory effects against five kinds of ginseng pathogens by confront culture.Among them,the highest inhibition rate to mycelia growth of Alternaria panax,reached 30.80%,followed by Rhizoctonia solani,Sclerotinia schinseng,Fusarium solani and Botrytis cinerea;On the PDA plate mixed with Chaetomium globosum FS-01 fermentation broth,the highest inhibition rate to mycelia growth of Botrytis cinerea,reached 82.09%,followed by Sclerotinia schinseng,Alternaria panax,Rhizoctonia solani and Fusarium solani;On the PDA plate mixed with Chaetomium globosum FS-01 conidia suspension,the highest inhibition rate to mycelia growth of Alternaria panax,reached 83.72%,followed by Botrytis cinerea,Rhizoctonia solani,Sclerotinia schinseng and Fusarium solani.Endophytic fungi FS-01 strain had a significant inhibitory effects,it could be utilized as a biological control resource inhibiting ginseng pathogens.