云南兔儿风的化学成分研究

Chemical constituents from Ainsliaea yunnanensis

为研究我国特有植物云南兔儿风的化学成分,该文采用常压正相硅胶色谱、凝胶色谱、低压氰基液相色谱和反相高压液相色谱等方法,对其乙醇提取物进行分离纯化并进行结构鉴定。结果表明:首次从云南兔儿风中分离并鉴定了10个化合物,包括6个甾体类、3个苯丙酸类和1个酚酸类,分别为3β-羟基豆甾-5-烯-7-酮(1)、3β-羟基豆甾-5,22-二烯-7-酮(2)、β-谷甾醇(3)、豆甾醇(4)、β-胡萝卜苷(5)、豆甾醇-3-O-葡萄糖苷(6)、3,5-O-二咖啡酰奎尼酸(7)、3,5-O-二咖啡酰奎尼酸甲酯(8)、咖啡酸(9)、原儿茶酸(10)。其中,化合物2和8是在兔儿风属中首次发现,所有化合物都是从云南兔儿风中首次发现。以上化合物的发现丰富了云南兔儿风的化学成分,为其合理、有效的应用提供了理论依据。

In order to study the chemical constituents,the ethanol extract of Ainsliaea yunnanensis was isolated and purified by the normal pressure silica column chromatography,Sephadex LH-20,the low pressure cyano chromatography and preparative high pressure liquid chromatography.Ten compounds were isolated and identified as 3β-hydroxystigmast-5-en-7-one(1),3β-hydroxystigmast-5,22-dien-7-one(2),β-sitosterol(3),stigmasterol(4),β-daucosterol(5),stigmasterol glucoside(6),3,5-O-dicaffeoylquinic acid(7),3,5-O-dicaffeoylquinic acid methyl ester(8),caffeic acid(9),protocatechuic acid(10).All compounds are isolated from this plant for the first time,and compounds 2 and 8 are isolated from this genus for the first time.The discovery of the compounds may enrich the chemical constituents of A.yunnanensis and provide a theoretical basis for its rational and effective application.