摘要
为探讨超声波辅助提取黑老虎叶总黄酮的最佳提取工艺条件及其抗氧化活性,该文以黑老虎叶为研究对象,采用超声波提取法提取黑老虎叶总黄酮,通过单因素试验研究提取时间、乙醇浓度、提取温度、料液比对黑老虎叶总黄酮提取率的影响,在单因素试验的基础上,采用正交试验优化其提取工艺条件,测试了最优条件下提取的黑老虎叶总黄酮对DPPH自由基、·OH自由基及超氧阴离子的清除能力。结果表明:黑老虎叶总黄酮超声辅助提取最佳提取条件为提取时间35 min、乙醇浓度80%、提取温度50℃、料液比1∶20 g·mL^-1,最佳条件下提取率为4.83%。抗氧化活性测试结果显示,黑老虎叶总黄酮表现出较好的清除DPPH自由基、·OH自由基及超氧阴离子能力,其抗氧化能力为清除DPPH自由基能力>清除超氧阴离子能力>清除·OH自由基能力。在浓度为0.8 mg·mL^-1时,黑老虎叶总黄酮清除DPPH自由基、·OH自由基及超氧阴离子的能力相当于同浓度下Vc的97.6%、82.1%、95.5%,黑老虎叶总黄酮是天然抗氧化剂的良好来源。上述结果为黑老虎叶活性成分的提取及开发利用提供了理论基础。
In order to study the optimum extraction conditions and antioxidant activity of total flavonoids from Kadsura coccinea leaves assisted by ultrasound extraction method,the ultrasonic-assisted extraction method of total flavonoids from K.coccinea leaves was studied,the influence factors of extraction time,ethanol concentration,extraction temperature and solid-liquid ratio on the extraction rate of total flavonoids from K.coccinea leaves were studied by single factor experiment,and orthogonal experiment was used to optimize the extraction process.The scavenging effects of total flavonoids extracted from K.coccinea leaves on DPPH,·OH and superoxide anion were tested under the optimum conditions.The results showed that the optimal extraction conditions were as follows:extraction time 35 min,ethanol concentration 80%,extraction temperature 50℃and solid-liquid ratio 1∶20 g·mL^-1,the maximum yield was 4.83%.The antioxidant activity test showed that total flavonoids of K.coccinea leaves had strong scavenging abilities on DPPH free radicals,·OH free radicals and superoxide anions.The order of antioxidant activities of total flavonoids from K.coccinea leaves was as follows:DPPH free radicals scavenging ability>superoxide anions scavenging ability>·OH scavenging ability.At the concentration of 0.8 mg·mL^-1,the scavenging abilities of total flavonoids from K.coccinea leaves on DPPH,·OH free radicals and superoxide anions were equivalent to 97.6%,82.1%and 95.5%of Vc at the same concentration,respectively.Total flavonoids of K.coccinea leaves were good sources of natural antioxidants.This study provides theoretical information for the extraction and utilization of active ingredients from K.coccinea leaves.
作者
李亚军
黄国保
全海燕
易鹊
谭明雄
LI Yajun;Huang Guobao;QUAN Haiyan;YI Que;TAN Mingxiong(College of Medicine&Technology,Hunan Polytechnic of Environment and Biology,Hengyang 421005,Hunan,China;Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology,College of Chemistry and Food Science,Yulin Normal University,Yulin 537000,Guangxi,China)
出处
《广西植物》
CAS
CSCD
北大核心
2020年第9期1381-1388,共8页
Guihaia
基金
广西自然科学基金(2017GXNSFBA198211,2018GXNSFAA294064)
广西农产资源化学与生物技术重点实验室开放基金(2019KF09)
湖南省职业院校教育教学改革研究项目(ZJGB2019149)
衡阳市2019年指导性项目(111)
湖南环境生物职业技术学院院长青年基金课题资助项目(Z2015-09)
湖南环境生物职业技术学院支柱工程项目
湖南省教育厅科学研究项目(19C0648)。
关键词
黑老虎叶
总黄酮
超声波辅助提取
工艺优化
抗氧化活性
Kadsura coccinea leaves
total flavonoids
ultrasonic-assisted extraction
technology optimization
antioxidant activity