木瓜蛋白酶分离蛋壳壳膜研究

Study on the Separation of Eggshell Membrane by Using Papain

介绍了利用木瓜蛋白酶酶解分离蛋壳壳膜的方法,探讨了木瓜蛋白酶酶解分离壳膜的最佳反应条件。结果表明,木瓜蛋白酶酶解分离壳膜的最佳反应条件如下:蛋壳粉用量为50 g,木瓜蛋白酶用量占蛋壳粉量的2.5‰,反应时间为90 min,反应温度为60℃,反应体系pH为5.5,反应体系固液比为1∶8。在此最佳反应条件下,回收壳下膜的平均重量为1.65 g。

This paper introduced the method of separating shell membrane by enzymatic hydrolysis of papain.The optimal reaction conditions for the separation of shell membrane by enzymatic hydrolysis of papain were as follows:eggshell powder dosage of 50 g,the proportion of papain dosage in eggshell powder dosage of 2.5‰,the reaction time of 90 min,the reaction temperature of 60℃,pH of the reaction system of 5.5,solid-liquid ratio of 1∶8.Under the optimal reaction conditions,the average weight of the under-shell membrane recovered 1.65 g.